东方百合Tiger Woods离体快繁技术体系的建立

Establishment of an in vitro Rapid Propagation System in Oriental Lily Tiger Woods

为探讨东方百合新品种Tiger Woods的组培快繁技术,以其花器官为外植体获得无菌试管苗,以无菌苗鳞片和叶片为次级外植体诱导不定芽形成,通过扩繁、生根获得完整植株,炼苗后移栽。结果表明:花梗与花丝诱导能力较强,其最适诱导培养基分别为MS+6-BA 2.0mg·L^(-1)+NAA 0.3mg·L^(-1)与MS+6-BA 1.0mg·L^(-1)+NAA 0.3mg·L^(-1)。无菌苗鳞片、叶片直接诱导产生不定芽的最适培养基为MS+TDZ 2.0mg·L^(-1)+NAA 0.1mg·L^(-1)与MS+TDZ 1.0mg·L^(-1)+NAA 0.5mg·L^(-1),诱导率为100%和93.33%,诱导系数为4.48和2.88;而上述两种外植体间接诱导愈伤组织的最适培养基为MS+PIC 1.0mg·L^(-1)+TDZ 0.5mg·L^(-1)与MS+PIC 1.0mg·L^(-1)+NAA0.5 mg·L^(-1),愈伤诱导率为93.33%和96.67%,分化系数为4.53和3.63。小鳞茎膨大最适培养基为MS+蔗糖75g·L^(-1),生根最佳培养基为MS+IBA 0.5mg·L^(-1),移栽最佳基质为草炭:蛭石:珍珠岩=1∶1∶1,成活率达91.11%。

In order to study the technology of tissue culture and rapid propagation on oriental lily new hybrids Tiger Woods, floral organs were used to establish the technology of tissue culture and rapid propagation. Scales and leaves excised of in vitro stock bulblets were used for inductions of adventitious shoots. The shoots were rooted to obtain plantlets. Finally plants regenerated from bulblet scales and leaves were successfully transferred to cultivation substrates. The results showed that the best shoots induction medium for pedicel was MS＋6-BA 2.0 mg·L-1＋NAA 0.3 mg·L-1, while for filament was MS＋6-BA 1.0mg·L-1 ＋ NAA 0.3 mg·L-1. For scales and leaves of in vitro stock bulblets, the optimum shoots inducement medium was MS＋TDZ 2.0 mg·L-1＋ NAA 0.1 mg.L-1 and MS＋TDZ 1.0 mg.L-1＋NAA 0.5 mg.L-1, in which the average induction rate was 100.00% and 93.33%, and the induction factor was 4.48, 2.88, respectively; while the best induction medium for callus induction was MS＋PIC 1.0 mg·L-1 ＋ TDZ 0.5 mg·L-1 and MS＋PIC 1.0 mg.L-1＋NAA 0.5 mg·L-1, in which the induction rate of callus was 93.33% and 96.67%. MS＋ sucrose 75 g·L-1 was appropriate for bulblets enlargement, MS＋IBA 0.5 mg·L-1 was best for rooting, the regenerated plantlets survived of Tiger Woods with highest rate of 84.00%, in substrate of peat： vermiculite： perlite=1： 1： 1.