山奈酚对慢性脑缺血大鼠学习记忆能力的影响及机制探讨

Effects of kaempferol on learning and memory in rats with chronic cerebral ischemia and possible mechanisms

目的探讨山奈酚(kaempferol,KAE)对慢性脑缺血大鼠的作用及其机制。方法采用双侧颈总动脉永久性结扎(bilateral common carotid arteries occlusion,2VO)建立大鼠慢性脑缺血动物模型;Morris水迷宫、抓握实验检测大鼠行为学;尼氏染色及HE染色观察脑组织病理改变;比色法检测MDA含量和SOD活性;Western blot检测脑组织DJ-1蛋白表达水平。结果与2-VO模型组比较,KAE明显改善慢性脑缺血诱导的学习记忆障碍和抓握能力损伤。此外,KAE明显减轻2VO大鼠脑组织病理损伤,增高脑组织中SOD活性,提高海马和皮层中抗氧化蛋白DJ-1的表达水平。结论KAE明显改善慢性脑缺血诱导的大鼠认知功能障碍、四肢平衡能力障碍及病理损伤,其机制可能与提高体内抗氧化系统有关。

Aim To investigate the effects and possible mechanisms of kaemperol in the rats with chronic cerebral ischemia. Methods Chronic cerebral hypoperfusion model was produced by permanent occlusion of bilateral common carotid arteries（ 2VO） in rats. After KAE treatment,the rats underwent Morris water maze and prehensile traction test. Neuronal morphology was observed using Nissl and HE staining. The activity of SOD and the content of MDA in brain tissue were determined. The DJ-1 protein expression was assayed by Western blot. Results Compared with 2VO model group,KAE significantly improved learning and memory and the grasping ability. In addition,KAE significantly reduced brain tissue pathological injury induced by 2VO. Furthermore, KAE significantly increased SOD activity and enhanced antioxidant protein DJ-1 expression in brain tissue. Conclusions KAE could significantly attenuate the cognitive impairment,limb balance dysfunction and pathological injury in rats with chronic cerebral ischemia. The mechanism may be related to improving the antioxidant system in vivo.