大肠癌肝转移患者血清中相关癌基因甲基化水平的检测

Detection of related oncogenes methylation in serum of colorectal cancer patients with liver metastasis

目的:探讨大肠癌肝转移患者血清相关癌基因甲基化水平的变化。方法:选择2011年9月—2013年12月因原发性大肠癌入院伴或不伴肝转移患者各32例,分别作为试验组A和试验组B。所有患者在入院之前均未进行化疗或放疗。另选择20例健康体检者作为对照组。提取研究对象血清中DNA,采用甲基化特异性PCR法检测E-cad、ID4、ESR1、APC或HACE基因甲基化异常。结果:试验组A检测出19例E-cad、ID4、ESR1、APC或HACE甲基化异常;试验组B检测出10例Ecad、ID4、ESR1、APC或HACE甲基化异常;对照组未检测出E-cad、ID4、ESR1、APC或HACE甲基化异常。3组间差异有统计学意义(P<0.01)。结论:血清E-cad、ID4、ESR1、APC和HACE基因甲基化状态联合检测有利于大肠癌肝转移的临床诊治。

Objective ： T o explore th e d etection results of serum related gene m ethylation in colorectal cancer patientsw ith liver m etastasis. Methods ： F ro m hospitalized patients w ith prim ary colorectal cancer from Sep tem ber 2011 to D ecem ber2 0 1 3 , 32 colorectal cancer patients w ith liver m etastasis and 32 colorectal cancer p atients w ithout liver m etastasis w ere selectedas the experim ental group A （ w = 3 0 ） and experim ental group B （ w = 3 0 ） , respectively. A ll patients did n ot undergochem otherapy or radiotherapy before ad m ission In addition, 20 healthy su bjects w ere recruited as th e control group. D N A wasextracted from seru m , and E -ca d , ID 4 , E S R 1 , A P C , and H A C E gene m ethylation w ere detected by m ethylation specific P C Rmethod. Results： 19 cases w ith E -ca d , ID 4 ,E S R 1 , A P C or H A C E abnorm al m ethylation w ere detected in the experim entalgroup A , 10 cases w ere detected in the experim ental group B , no case was detected in th e control group. T h e re w ere significantdifferences am ong the th ree groups （P 〈 C 0 . 0 1 ） . Conclusions： D etection of serum E -c a d ,ID 4 ,E S R 1 ,A P C , H A C E genem ethylation statu s is of g reat significance for clinical diagnosis and treatm en t of colorectal cancer liver m etastasis.