响应面法优化毒三素链霉菌中利普司他汀的提取工艺

Optimization of lipstatin extraction from Streptomyces to xytricini by response surface methodology

采用响应面法对毒三素链霉菌提取工艺进行优化。首先确立乙醇为最佳的溶剂。以利普司他汀提取收率为指标,以乙醇为浸提溶剂,对料液比、乙醇体积分数、浸提时间、浸提温度和浸提次数进行单因素实验。在此基础上,选取料液比、乙醇体积分数、浸提时间为自变量,采用Box-Behnken设计的方法,研究各自变量及其交互作用对利普司他汀提取收率的影响。结果表明:毒三素链霉菌中利普司他汀的最佳提取工艺条件为液料比6.32∶1 m L/g、乙醇体积分数95%、浸提时间3.61 h。在此条件下,利普司他汀提取收率的预测值为83.35%,实验验证值为84.50%,相对误差为1.36%。

Response surface methodology was used to optimize the extraction conditions of lipstatin from Streptomyces toxytricini. First , ethanol was established as the best solvent.The extraction yield of lipstatin was the in d ex ,ratio of solid to solvent,ethanol concentration,extraction time ,extraction temperature and extraction numbers were studied as factors by extraction solvent ethanol. Based on the single factor experiments,the effects of ratio of solid to solvent,ethanol concentration,extraction time ,as well as their interactions on extraction yield of lipstatin were studied by Box-Behnken design.The optimal extraction conditions of lipstatin from Streptomyces toxytricini were as follows ： the ratio of solid to solvent was 6.32： 1 mL/g,ethanol concentration was 95%,extraction time was 3.61h .Under these conditions,the model- predicted and experimental values of lipstatin yield were 83.35% and 84.50% , respectively, revealing 1.36% relative error between them.