原发性胆汁性肝硬化患者IgG型自身抗体检测的临床价值

The clinical value of IgG autoantibodies detection in patients with primary biliary cirrhosis

目的分析原发性胆汁性肝硬化(PBC)患者的自身免疫性肝病抗体谱及抗核抗体荧光核型,探讨其在PBC诊断及临床应用中的意义。方法选择已确诊的PBC患者67例及病例对照组70例,检测自身免疫性肝病抗体谱,间接免疫荧光法检测抗核抗体,应用SPSS17.0统计软件进行分析处理。结果 PBC患者组阳性检出率以AMA-M2抗体、Sp100抗体及gp210抗体为主,抗核抗体荧光核型以核点型和胞浆颗粒型为主。AMA-M2抗体、Sp100抗体和gp210抗体在PBC患者的阳性率显著高于对照组(χ2=37.228,5.492和16.292,P<0.01);而LKM1抗体、LC1抗体、SLA抗体阳性率与对照组比较,差异无统计学意义(P>0.05)。结论自身免疫性肝病抗体谱,特别是血清AMA-M2阳性是诊断PBC的重要指标之一,另外Sp100抗体、gp210抗体也是PBC诊断的特征性抗体,其对AMA-M2阴性PBC的诊断具有重要作用。

Objective To analyze the spectrum of autoimmune liver disease in patients with primary biliary cirrhosis（PBC）and to investigate the clinical significance of PBC in the diagnosis and clinical application.Methods Totally 67 cases of PBC and 70 cases of disease control were selected as the research subjects.Detection of anti nuclear antibodies and indirect immunofluorescence detection of anti nuclear antibodies,using SPSS17.0statistical software for analysis and processing.Results The positive detection rate of PBC patients was mainly AMA-M2 antibody,Sp100antibody and gp210 antibody,and ANA fluorescent nuclear type was mainly nuclear and cytoplasmic particle type.The positive rates of AMA-M2,anti Sp100 antibody and anti gp210 antibody in PBC patients were significantly higher than those in the control group（χ2=37.228,5.492 and 16.292,P〈0.01）.In comparison with the control group,there was no significant difference in the positive rate of LKM1 antibody,LC1antibody and SLA antibody（P〉0.05）.Conclusion Antibody spectrum of autoimmune liver disease,especially the AMA-M2 positive serum is one of the important indicators in the diagnosis of PBC,in addition,Sp100 antibody,gp210antibody is also the characteristic of PBC diagnosis antibody,which has an important role in the diagnosis of AMA-M2 negative PBC.