转AtHDG11基因玉米的获得及抗旱性鉴定

Expression of AtHDG11 in Transgenic Maize and Drought Resistance Identification

培育抗除草剂和抗旱的转基因玉米种质,通过超声波辅助农杆菌介导花粉非组培法将拟南芥抗旱基因At HDG11导入玉米自交系昌7-2中,获得转基因植株。通过逐代除草剂筛选、分子检测对获得的转基因植株进行检测,获得5株转基因株系。在此基础上,以非转基因自交系昌7-2为对照,对5个转基因株系进行抗旱性鉴测;对苗期和十叶期玉米植株进行干旱胁迫处理,测定转基因植株生理活性物质和光合参数。结果表明,在干旱胁迫条件下,转基因玉米叶片的Pro含量高于非转基因玉米,MDA含量低于非转基因玉米;不论在正常生长条件下还是干旱条件下,转基因株系的光合速率和水分利用率都明显高于对照植株;而转基因株系的蒸腾速率和气孔导度则明显低于对照植株。综合室内与田间的抗旱检测结果表明,超声波辅助农杆菌介导花粉的非组培转基因方法在玉米上是可行的。将拟南芥HDG11基因导入玉米,同样可以有效地提高玉米的抗旱性,为玉米的抗旱育种提供新的种质资源和新的转基因方法。

The objective of this experiment is to study the production of transgenic maize with the resistance of drought stress.In this study,an expression vector containing drought resistance gene At HDG11 was used to transform to maize elite inbred line Chang 7-2 by ultrasonic assisted Agrobacterium mediated pollen transformation method,and produced transgenic maize plants.Based on herbicid resistance,PCR analysis and transcriptional analysis,five transgenic maize lines were selected from a large number of transgenic lines.Subsequently,the inbred line Chang7-2 was used as the nontransgenic control to analyze the drought resistance of transgenic plants.Physiological active substances and photosynthetic parameters were measured on plants at the seedling stage and 10-leaf stage in a drought stress treatment.The results showed that Pro content of transgenic maize was higher than that of the CK plants under drought stress conditions,while MDA content was lower.Both normal conditions and drought condition,photosynthetic rate and water use efficiency of transgenic lines were significantly higher than that of CK plants.And transpiration rate and stomatal conductance of transgenic lines were significantly lower than the control plants.Therefore,ultrasonic assisted Agrobacterium mediated pollen transformation method is feasible in the maize.At HDG11 is a strong biotechnological candidate for use in efforts to produce a drought-resistance maize.This study will provide new germplasm resources and new transgenic method.