CD147在不明原因自然流产患者绒毛组织的表达

Expression of CD147 in villous tissues of patients with unexplained spontaneous abortion

目的:分析CD147在不明原因自然流产患者绒毛组织中的表达水平,并探讨其生物学功能。方法:收集人早孕期正常和不明原因自然流产妇女的绒毛组织,采用免疫组织化学、RT-PCR和Western blotting法检测CD147的表达,比较CD147在正常早孕与不明原因自然流产妇女绒毛组织的表达差异。体外分离纯化获得正常人早孕期滋养细胞,采用免疫细胞化学验证CD147在滋养细胞的表达,引入抗CD147中和性抗体处理人滋养细胞,采用Brd U增殖实验和Transwell侵袭实验分析滋养细胞增殖和侵袭能力的变化。结果:正常早孕期绒毛组织和滋养细胞高表达CD147,不明原因自然流产绒毛组织CD147的mRNA和蛋白表达水平均低于正常早孕期绒毛组织。抗CD147中和性抗体处理原代人滋养细胞后,滋养细胞的增殖能力未发生明显变化,侵袭能力下降。结论:人绒毛组织CD147的异常低表达可能与不明原因早期妊娠失败相关,CD147通过调控滋养细胞的侵袭能力参与正常妊娠的维持。

AIM： To analyze the expression levels of CD147 in villous tissues of patients with unexplained spontaneous abortion and to investigate its biological function in human primary trophoblasts in vitro. METHODS： The villous tissues were collected from the women with normal early pregnancy and unexplained spontaneous abortion. The expression levels of CD147 were determined by immunohistochemical staining,reverse transcriptase-polymerase chain reaction（ RT-PCR） and Western blotting. The primary trophoblasts were prepared by the trypsin-DNase I digestion and cultured in vitro. CD147 expression in the primary trophoblasts was detected by immunocytochemical staining. The primary trophoblasts were treated with anti-human CD147 neutralizing antibody,and then the cell proliferation and invasion abilities were evaluated by Brd U incorporation assay and Matrigel-coated Transwell assay,respectively. RESULTS： CD147 was expressed in the first-trimester villous tissues and human primary trophoblasts. The mRNA and protein levels of CD147 were lower in the villous tissues from the patients with unexplained spontaneous abortion than those from the women with normal pregnancies. The trophoblast invasion decreased significantly accompanied with no evident change in proliferation after treatment with anti-human CD147 neutralizing antibody. CONCLUSION： The abnormal low expression of CD147 in human villous tissues may correlate with early unexplained spontaneous abortion. CD147 plays a role in the regulation of trophoblast invasion and participates in the maintenance of successful pregnancy.