糖肾方对糖尿病肾病大鼠血液流变学及内皮细胞功能的影响

Influence of Tangshen formula on endothelial function and hemorheology in diabetic nephropathy rats

目的:观察糖肾方对糖尿病肾病(DN)大鼠血液流变学及内皮功能的影响。方法:采用高脂高糖饮食联合链脲佐菌素(STZ)诱导建立2型糖尿病合并DN大鼠模型,将建模成功大鼠随机分为DN模型组、糖肾方组和缬沙坦组,同时设正常对照组。药物干预12周后,全自动生化分析仪检测大鼠空腹血糖(FBG)、血清总胆固醇(TC)和甘油三酯(TG)水平;微循环血流仪检测肾微循环变化;血液流变仪检测血液流变学指标;ELISA技术检测尿白蛋白以及血清假血友病因子(v WF)和纤溶酶原激活物抑制剂-1(PAI-1)含量。结果:与正常对照组比较,DN模型组大鼠FBG、血清TC、TG和尿白蛋白明显升高(P<0.05),血清v WF浓度升高(P<0.01);与DN模型组比较,糖肾方组和缬沙坦组的FBG差异无统计学显著性,血清TC、TG和尿白蛋白明显降低(P<0.05),v WF降低(P<0.01)。与正常对照组比较,DN模型组的血浆黏度,卡松黏度,全血高切、中切黏度,红细胞聚集指数,刚性指数及电泳时间均显著升高,红细胞变形指数降低(P<0.01)。与DN模型组比较,治疗组的血浆黏度,卡松黏度,全血高切、中切和低切黏度,红细胞聚集指数,刚性指数及电泳时间均明显降低(P<0.01),红细胞变形指数升高,但无显著性差异。与正常对照组比较,DN模型组的肾皮质微循环血流量减少(P<0.01)。与DN模型组比较,糖肾方组的肾皮质微循环明显增加(P<0.05),缬沙坦组无明显改变。结论:糖肾方能降低实验性DN大鼠的血脂水平,改善血液流变性和内皮功能,从而改善微循环,对糖尿病肾脏具有一定的保护作用。

AIM： To explore the influence of Tangshen formula（ TS） on endothelial function and blood rheology in diabetic nephropathy（ DN） rats. METHODS： The DN rat model was established by intravenous injection of lowdose（ 30 mg / kg） streptozotocin（ STZ） after having the high-fat / high-glucose diets for one month. The animals were divided into DN model group,TS group and valsartan group. Fasting blood glucose（ FBG）,serum total cholesterol（ TC）,serum triglyceride（ TG）,renal cortex blood flow and hemorheology were monitored. The content of von Willebrand＇s factor（ v WF） and plasminogen activator inhibitor-1（ PAI-1） in the serum was determined by ELISA. RESULTS： Compared with normal group,FBG,TC,TG,v WF and PAI-1 were increased in DN model group（ P〈0. 01）. Compared with DN model group,TC,TG,v WF and PAI-1 were decreased in TS group and valsartan group（ P〈0. 05）,and no significant difference of FBG was observed. Compared with normal group,plasma viscosity,Casson viscosity,whole blood high / medium / low-shear viscosity,erythrocyte aggregation index,erythrocyte rigidity index and erythrocyte electrophoresis time were increased,and erythrocyte deformation index was decreased in DN model group（ P〈0. 01）. Compared with DN model group,plasma viscosity,Casson viscosity,whole blood high / medium / low-shear viscosity,erythrocyte aggregation index,erythrocyte rigidity index and erythrocyte electrophoresis time were decreased（ P〈0. 05）,but there was no significant difference for erythrocyte deformation index in TS group. Compared with normal group,the renal cortex microcirculation blood flow in DN model group was significantly decreased. Compared with DN model group,the renal cortex microcirculation blood flow was significantly increased in TS group（ P〈0. 05）,and no significant change in valsartan group wasfound. CONCLUSION： Tangshen formula plays a protective role in the kidney of diabetic rats by improving the blood rheology and endothelial function,thus ameliorating the renal cortex microcirculation blood flow in experimental diabetic rats.