基于乙烯基砜化学的高分子膜表面化学糖基化及其活性研究

Preparation and bioactivity study of glycosylated polymer film based on vinyl sulfone chemistry

在高分子膜表面通过化学方法构建糖基化层,实现对生物膜致密"糖被"层的仿生模拟,可以充分拓展及发挥膜材料和糖的生物学功能。利用二乙烯基砜与羟基反应的特性,以乙烯基砜为偶联剂将甘露糖接枝到聚甲基丙烯酸-2-羟乙酯(PHEMA)聚合物膜表面,制备甘露糖糖基化PHEMA膜。利用傅里叶变换红外光谱对糖基化膜结构进行了表征,根据血凝素蛋白特异性识别糖的性质对糖基化反应条件进行了优化,并在蛋白和细胞水平对其进行了生物学评价。结果表明,制备的甘露糖糖基化PHEMA膜具有良好的抗垢性能,能够特异性识别和吸附伴刀豆球蛋白A(ConA);糖基化膜能够引起小鼠单核巨噬细胞(RAW 264.7)特异性粘附且具有较低的细胞毒性。提供了一种高分子膜表面糖基化的简单方法,为仿生膜表面的构建以及细胞表面糖参与的生物代谢活动的研究提供了基础。

Glycosylation of polymer film is essential for mimicking the dense “glycocalyx”in biomembrane and development of bio-functional materials.In this study,a convenient method was developed to conj ugate glycose onto poly（2-hydroxyethyl methacrylate）（pHEMA）film using divinyl sulfone（DVS）as the coupling agent.A mannose glycosylated pHEMA film was prepared and characterized by Fourier transform infrared spectroscopy （FT-IR）.The reaction conditions were optimized using specific protein adsorption studies,and the bioactivity of the glycosylated film was assessed at protein and cell level.The protein level results show that the mannose gly-cosylated pHEMA film has good anti-fouling capabilities and can specifically recognize Concanavalin A （ConA）. The cell adhesion experiment using macrophages （RAW 264.7）suggest that the glycosylated film is highly bio-active and low cytotoxic.The glycosylation of polymer film using DVS provides a neat approach for the con-struction of biomimetic membrane and researches on glycometabolism process at cell level.