乙醛脱氢酶2基因多态性与酒精性肝病的相关性研究

Correlation study between polymorphism of aldehyde denydrogenase 2 gene and alcoholic liver disease

目的探讨酒精性肝病(alcoholic liver disease,ALD)与乙醛脱氢酶2(aldehyde dehydrogenase 2,ALDH2)基因多态性的相关性。方法收集87例长期饮酒患者,其中44例为ALD患者,43例为长期嗜酒非酒精性肝病(non-ALD,NALD)患者,30名健康对照者,抽取其外周血标本提取DNA,DNA微阵列芯片法对ALDH2进行基因分型,分析ALDH2基因型与ALD的关系。结果 ALDH2各基因型频率在ALD组与健康对照组中分布差异有显著统计学意义(P<0.01),嗜酒NALD组与健康对照组中ALDH2基因频率分布差异无显著统计学意义(P>0.01);ALDH2基因多态性与饮酒后发生潮红综合征相关,与GGT、AST有相关性。结论乙醛脱氢酶不同基因型与ALD的发生有一定相关性。

Objective To investigate the correlation between the alcoholic liver disease（ALD） and polymorphism of aldehyde dehydrogenase 2 gene（ALDH2）. Methods Eighty-seven cases of chronic alcohol drinking history patients were collected,including 44 cases of ALD,43 cases of long-term drinking NALD patients. In addition,30 cases of healthy control subjects were selected. DNA was extracted from peripheral blood samples. And DNA microarray chip was carried out to analyze the correlatior between ALDH2 genotype and ALD. Results Different genotypes frequency distribution of ALDH2 had a statistically significant difference between ALD group and healthy control group（P〈0. 01）. However,there was no statistically significant difference between long-term drinking NALD group and healthy control group（P〉0. 01）. ALDH2 genotypes were associated with alcohol flushing symptoms occurred after drinking,and they were related to GGT and AST. Conclusion There is a certain correlation between the different genotypes of ALDH and the occurrence of ALD.