基于微卫星分子标记的云南松及其近缘种遗传关系分析

Genetic Relationship of Pinus yunnanensis with Related Pine Species Based on Microsatellite Molecular Markers

采用SSR分子标记技术,对云南松分布区内或相邻地区的松属双维管束亚属3种、3变种及扭曲的云南松共7个种或变种(变型)进行遗传分析。利用9对SSR引物,从7个种或变种(变型)100份材料中检测出31个等位基因,单个位点的等位基因数为2~6个,平均3.4个;思茅松群体遗传多样性较高,而细叶云南松较低。基于Nei's遗传距离利用非加权配对算术平均数法(UPGMA)聚类,云南松等7个种、变种(变型)可以分成3大类,其中马尾松和思茅松各自独立成为一类,其余5个种、变种(变型)聚为一类,以云南松与扭松的遗传关系最近,无明显的遗传分化,它们的表型变异可能由人为生产活动的负向选择或环境恶化引起。

Detected the genetic relationship of 7 species or varieties （forma） belong to the genus Pinus including 3 species, 3 varieties and crooked P.yunnanensis, with SSR molecular marking technology in distribution regions or neighboring regions of P.yunnanensis. was 100 individuals of 7 species or varieties （forma） presented 31 alleles with 9 pairs of SSR primers. The number of alleles varied 2 to 6 with an average of 3.4 for each locus. There were high genetic diversity in P.kesiya var. langbianensis population and low genetic diversity in P.yunnanensis var. tenuifolia. Those species or varieties （forma） were classified into 3 main groups by UPGMA cluster analysis based on Nei′s （1983） genetic distance. Among them, P.massoniana and P.kesiya var. langbianensis classified as a separate group, respectively, the other 5 species or varieties （forma） clustered into 1 group. The most similar ones were found between P.yunnanensis and crooked P.yunnanensis and obvious genetic differentiation was not found. The phenotypic degeneration may be caused by the artificial negative selection or environmental degradation.