贵州南部野生兜兰SRAP遗传多样性分析

Genetic Diversity Analysis of Wild Paphiopedilum Species in the South of Guizhou

利用SRAP分子标记对贵州南部地区8个野生兜兰的遗传多样性进行分析,从合成的360对引物中筛选出8对多态性丰富、重复性好且扩增条带清晰的引物分别对野生兜兰DNA进行扩增,建立野生兜兰最适的PCR扩增反应体系,并利用UPGMA法对野生兜兰进行亲缘关系的聚类分析。结果表明:PCR反应共扩增出152个条带,其中多态性条带122条,多态性位点百分率为80.26%;平均观察等位基因数(Na)为1.763 2,平均有效等位基因数(Ne)为1.506 1,Nei's基因多样性指数(H)为0.287 9,Shannon信息指数(I)为0.424 1;供试材料的遗传相似性系数变化范围为0.513~0.756,以相似系数0.67为阀值,将不同来源野生兜兰分为3组,研究显示兜兰基于SRAP分子标记的聚类分析与形态学分类结果一致。

SRAP marker was used to detect the genetic diversity of 8 accessions of wild Paphiopedilum species. 8 pairs of polymorphic and repeatable primers were screened out for amplification and establishing the optimal PCR reaction system and genetic relationships of wild Paphiopedilum were detected by UPGMA cluster analysis. Totally 152 clear bands were revealed, including 122 polymorphic bands, and the percentage of polymorphic bands was 80.26%. The mean observed number of alleles （Na） was 1.763 2, the effective number of alleles （Ne） was 1.506 1, the Nei′s gene diversity index （H） was 0.287 9 and the Shannon information diversity index （I） was 0.424 1. The genetic identity coefficient was between 0.513 to 0.756. Divided wild Paphiopedilum from different sources into 3 groups with the threshold value of identity coefficient as 0.67, the study showed that the clustering of Paphiopedilum species based on the SRAP markers was consistent with that from morphological classification.