厚壁型和薄壁型巨龙竹的分子标记研究

Study on Thick-Walled and Thin-Walled Types of Dendrocalamus sinicus by Molecular Markers

采用RAPD技术对28株厚壁型和薄壁型巨龙竹样本进行遗传分化研究。结果表明:88条随机引物中筛选出17条重复性好、带型清晰明亮的引物,用于RAPD标记,共检测出131个位点,其中多态性位点118个,多态性程度为90.08%。薄壁型巨龙竹的多态性高于厚壁型巨龙竹,2种类型的遗传分化系数为0.154 6。UPGMA聚类分析显示大部分薄壁型和厚壁型巨龙竹各自聚成一类,证明厚壁型巨龙竹和薄壁型巨龙竹在DNA水平上存在明显差异。

Study on genetic differentiation of 28 accessions thick-walled and thin-walled Dendrocalcamus sinicus was anaylyzed by using RAPD markers. Out of 88 RAPD primers, 17 primers with good reproducibility and clear ribbon were selected for RAPD markers. 131 loci were identified totally, 118 of which were polymorphic. The ratio of polymorphism was 90.08%. Polymorphism of thin-walled type was higher than that of thick-walled type. The genetic differentiation coefficient of both types was 0.154 6. Most of the thin-walled and thick-walled types were respectively grouped into one class by cluster analysis （UPGMA）. It was proved that there were obvious differences between the thick-walled and the thin-walled D.sinicus in DNA.