魔芋葡甘聚糖与丝素肽的溶胶特性

Properties of the Konjac Glucomannan and Silk Fibroin Peptide Blend Sol

采用物理共混的方法制备不同配比的魔芋葡甘聚糖-丝素肽共混溶胶,通过流变仪分析共混溶胶的流变特性。经过脱胶、溶解、透析等3步骤纯化后进行检验,最后酶解得到丝素肽,并通过共混溶胶的粘度测试、振荡测试及动态力学分析等3个方面探索魔芋葡甘聚糖与丝素肽的溶胶特性。结果表明:该共混溶胶是一种假塑性流体,具有"剪切变稀"的特征,随着魔芋葡甘聚糖组份比例的增加,共混溶胶的粘度升高;当魔芋葡甘聚糖与丝素肽的摩尔质量比为2︰1时,弹性模量与损耗模量的交点处于最低频率处,该共混体系最稳定。

Konjac glucomannan/silk fibroin peptide blend sols were prepared at different ratios by using physical blending method,and their rheological properties were analyzed by using the rheometer. Silk fibroin peptide was extracted and purified from the silkworm cocoon after degumming,dissolution and dialysis and then prepared after enzymolysis. The silk fibroin peptide was used to blend with konjac glucomannan（ KGM） at different ratios to prepare KGM/silk fibroin peptide（ SP） sols,and the properties of the KGM/SP blend sols were then determined through viscosity test,shock test and dynamic analysis. The blend sols were a pseudo plastic fluid with shear thinning behavior. The viscosity of the blend sols increased with the proportion of the konjac glucomannan in the blend sols. The KGM/SP blend sols prepared at the volume ratio of 2 ︰ 1 had the lowest frequency at the intersection of the elastic modulus and the loss modulus and was hence the most stable.