肝炎病毒对外周淋巴细胞染色体畸变的研究

To study aberration induced by hepalitis virus in peripheral lymphocyte chromosome

探讨肝炎病毒对宿主外周淋巴细胞染色体致畸变以及染色体畸变与肝脏病变、病原复制指标之间的关系。抽取各临床期病毒性肝炎患者 6 4例外周血 ,肝素抗凝 ,培养 ,分离淋巴细胞 ,常规方法制备染色体片 ,做肝功能及病原学指标检验。观察染色体畸变率及畸变类型分析。 5 0例标本有染色体畸变 ,15例染色体畸变率≥ 3% ,16例染色体畸变率高达 4 %— 6 5 %。肝炎病毒感染可致较高淋巴细胞染色体畸变。

To explore the aberration induced by hepatitis in peripheral lymphocyte chromosome of hosts and the relations among chromosome aberration,liyer pathological changes,and the indices of pathogeny duplication.The peripheral blood in 64 cases of virus hepatitis,with different clinical stages was drawn,mixed with haparin,cultured and with lymphocyte separation.The chromosomal smear was made by routine methods,the liver function and pathogeny indices were examined.The rate and type of aberration of chromosome were observed.50 Samples showed chromosome aberration.The rate of chromosome aberration was ≥3% in 16 cases,and reached as high as 4%-6 5% in another 15 cases.The infection of hepatitis virus may cause lymphocyte chromosome aberration,and it seems that there were some relations between the degree of live damage and the indices of virus duplication.