SLE结合GC-MS法测定葡萄酒中氨基甲酸乙酯

Determination of ethyl carbamate in wine by SLE combined with GC-MS

建立了固液萃取(SLE)结合气相色谱-质谱联用(GC-MS)法测定葡萄酒中氨基甲酸乙酯(EC)含量的检测方法。以硅藻土为吸附剂,二氯甲烷为萃取溶剂,对葡萄酒中氨基甲酸乙酯进行萃取。结果表明,二氯甲烷用量为70 m L,酒样中乙醇体积分数低于12%,萃取效果最佳。EC在200~1 600μg/L范围内线性关系良好(R2=0.997 9)。加标回收率为91.30%~99.90%,相对标准偏差(RSD)为1.26%~1.98%,方法的精密度RSD值为1.61%~3.06%。表明该方法准确度高、重现性和精密度良好,适合用于葡萄酒样品中EC含量的检测。

The determination method of ethyl carbamate （EC） in wine was established by SLE combined with GC-MS. Using diatomite as adsorbent, dichloromethane as extraction solution, the EC in wine was extracted. The results showed that the optimum extraction conditions were as follow： dichloromethane dosage 70 ml, ethanol content in wine 〈 12%. EC had a good linear relationship in range of 200-1 600 μg/L （RZ=0.997 9）, the adding standard recovery rate was 91.30%-99.90%, and relative standard deviation （RSD） was 1.26%-1.98%. The RSD of method precision was 1.61%-3.06%. Due to high accurate, good reproducibility and accuracy, the method could be used for the determination of EC content in wine.