黄羽祖代公鸡精液作为禽白血病净化检测材料的应用研究

Applied Research of Yellow Feather Grandparent Roosters Semen as Test Sample in Avian Leukosis Eradication Program

为了解黄羽祖代公鸡精液在禽白血病(AL)净化中的作用,选取广东某黄羽祖代种鸡场a、b、c三个品系的公鸡作为研究对象,分别无菌操作逐一采集a、b、c三个品系公鸡的泄殖腔拭子及其相对应的血液与精液样品。采用ALV p27抗原ELISA的检测方法对泄殖腔拭子进行直接检测,用DF-1细胞分别对血浆和精液样品进行病毒分离,并将检测结果进行比较分析。结果显示:公鸡的泄殖腔拭子ALV p27抗原ELISA检测、血浆病毒分离为阴性的样品,从其对应的公鸡精液样品中能分离到外源性ALV,并且发现黄羽祖代公鸡精液阳性样品及其相对应的泄殖腔拭子阳性样品与血浆阳性样品相互之间不能完全覆盖;分别仅单独检测种公鸡的泄殖腔棉拭子、精液或血液中的ALV时存在漏检。因此,精液作为黄羽祖代公鸡禽白血病净化的检测材料是可行的,在针对黄羽祖代公鸡进行AL净化时,不应忽略精液样品的检测,为了提高检出率,加快净化进程,应考虑不同样品组合的复合检测。

In order to investigate the role of semen of yellow feather grandparent roosters in avian leukosis（AL）eradication process,a,b,c three different breeds of yellow feather grandparent roosters were chosen to be sampled.ALV p27 antigen ELISA was used in direct detection of cloacal swabs,while anti-coagulated blood and semen sample were used to isolate and detect exogenous ALV based on DF-1cells.Upon comparative analysis,the results showed that the virus could be isolated from semen,while the result of ALV p27 antigen ELISA S/P value of cloacal swabs and the virus isolation of plasma were negative.Three results couldn＇t completely correspond respectively.Individual AL deteetion of cloacal swabs,plasma or semen could lead to missed detection.Therefore in AL eradication of yellow feather breeds,semen as the test sampleis feasible and should not be neglected,multiple samples should be considered in order to improve the detection rate and speed up the purification process.