复方中药对肉鸡腹水综合征肺组织血管内皮细胞源性增殖因子的影响

The Effect of the Compound Traditional Chinese Medicine on Endothelium Derived Multiplication Factor in Lung of Broilers with Ascites Syndrome

旨在研究肉鸡腹水综合征发生发展过程中的肺组织EGFL7、VEGF和VEGFR2等血管内皮细胞源性增殖因子表达量的变化和复方中药对其影响。275羽8日龄罗斯肉鸡随机分为空白组(常规饲养),模型组(9~11℃,饲料中添加3%猪油和4%鱼粉,饮水中添加0.12%NaCl),复方中药高、中、低剂量组(饲养同模型组,每天于饮水中分别给予2、1、0.5mL·kg-1复方中药),用荧光定量PCR和ELISA检测15、25、35、45日龄肉鸡肺组织EGFL7、VEGF、VEGFR2的基因和蛋白质表达量。结果表明:与空白组相比,模型组肉鸡腹水心脏指数极显著升高(P<0.01),肺组织EGFL7、VEGF、VEGFR2的基因和蛋白质表达量显著增加(P<0.01或P<0.05);与模型组相比,复方中药高、中剂量组均能不同程度地改善肉鸡的精神状态、腹水、心包积液等症状,能显著下调腹水心脏指数和肺组织EGFL7、VEGF、VEGFR2的基因和蛋白质表达量(P<0.01或P<0.05)。腹水综合征肉鸡肺组织EGFL7、VEGF、VEGFR2的基因和蛋白质表达量升高,参与了肉鸡腹水综合征病理的发生发展;复方中药能下调其表达,保护肺组织血管内皮功能,有效防治肉鸡腹水综合征。

The objective of this study was to investigate the changes of EGFL7,VEGF and VEGFR2 in lung of broilers with ascites syndrome and analyze the influence of compound traditional Chinese medicine for endothelium derived multiplication factor on AS.A total of 275 8-day-old Ross broilers were randomly divided into five groups,blank group bred with basal feed,model group bred with mixed feed（9-11℃,added 3%lard and 4%fish meal to basal feed,added 0.12% NaCl to drinking water）,compound traditional Chinese medicine high,medium and low dose group bred with mixed feed and respectivelygiven a dose of 2,1,0.5mL·kg-1 in drinking water.Using real-time fluorescence quantitative PCR and ELISA to detect the content of EGFL7,VEGF and VEGFR2 in lung tissue.Compared with blank group,ascites heart index in model group were increased significantly（P〈0.01）,and relative expression levels of EFGL7,VEGF,VEGFR2 mRNA and protein expression levels in lung of broilers in model group were increased significantly（P〈0.01 or P〈0.05）;Compared with model group,compound traditional Chinese medicine high and medium dose group were able to improve clinical symptoms（mental state,ascitic fluid and hydropericardium）,compound traditional Chinese medicine could effectively down regulate gene and protein expression levels of EGFL7,VEGF and VEGFR2 in lung of broilers（P〈0.01 or P〈0.05）.The study showed the increases of gene and protein expression levels of EGFL7,VEGF and VEGFR2 in lung of broilers participated in the pathogenesis of AS.Compound traditional Chinese medicine can effectively prevent and treat of ascites syndrome by down regulate gene and protein expression levels of EGFL7,VEGF and VEGFR2 in lung of broilers to protect vascular endothelium.