利用代谢组学方法分析生防菌抑菌机理模式的初步建立

Establishment of a Analysis Method for Antifungal Modes of Biocontrol Strains on Cucumber Fusarium Wilt by Metabonomics

应用9种杀菌剂来处理尖孢镰刀菌,通过HPLC/Q-TOF方法进行代谢轮廓分析。结果表明:不同菌剂处理后的尖孢镰刀菌代谢组显著差异,PCA分析可将其分为6组。将6组杀菌剂作为训练样本集,建立杀菌剂对尖孢镰刀菌抑菌机理的PLS-DA模型。以枯萎病生防菌短短芽胞杆菌为测试样本,利用该模型对其活性物质进行了抑菌机理的分析判别,发现其作用方式与嘧菌酯、氰霜唑类似,即通过抑制病原菌的呼吸作用而发挥抑菌功能,说明利用该模型判别生防菌对尖孢镰刀菌的抑菌机理是可行的。

In this investigation, the antifungal modes of action of nine fungicide with different targets or modes- of-action on Fusarium oxysporum were studied. Metabolic profiles of cultures treated with nine different fungicide were acquired by LC/Q-TOF MS, significant metabolic markers were used for classification by PCA analysis, and the fungicide could be divided into six groups. Using the six groups as training sample set, PLS-DA prediction model were established. Confirmed with the model, the conclusion could be drawn that the antifungal mode of action of Brevibacillus brevis, a bioeontrol strain of Cucumber Fusarium Wilt, is similar to that of azoxystrobin and Cyazofamid, which act as respiratorv inhibitor.