桦木酸对人肝癌HepG2肿瘤干细胞增殖的影响

Effects of Betulinic Acid on Proliferation of Human Liver Cancer HepG2 Cells

目的观察桦木酸(betulinic acid,BA)对人肝癌HepG2肿瘤干细胞增殖的影响,并从细胞生长周期和细胞凋亡两方面初步探讨其抗肝癌的作用机制。方法体外培养人肝癌HepG2肿瘤干细胞并证明其自我更新能力。CCK-8法检测40、20、10、5、2.5、1.25μmol/L BA作用人肝癌HepG2肿瘤干细胞24、48 h的细胞活力;40、20、10、5μmol/L BA作用人肝癌HepG2肿瘤干细胞48 h,流式细胞术检测细胞周期和细胞凋亡率。结果 BA对人肝癌HepG2肿瘤干细胞增殖有明显的抑制作用,并呈一定的量-效关系;BA能明显影响肿瘤干细胞周期,诱导肿瘤干细胞凋亡,40μmol/L BA明显阻滞细胞周期于S期,且细胞凋亡率达到10.86%。结论 BA对人肝癌HepG2肿瘤干细胞增殖具有明显的抑制作用,其可能通过影响肿瘤干细胞周期及诱导肿瘤干细胞凋亡而发挥作用。

Objective To observe the effects of betulinic acid（BA） on proliferation of human hepatoma stem cell; To discuss its anti-cancer mechanism from the aspects of cell cycle and cell apoptosis. Methods Hep G2 stem cells were cultivated in vitro and testified the self-renewal capacity. The effects of BA in concentration of 40, 20, 10, 5, 2.5, 1.25 μmol/L on the cell vitality of cultured human liver cancer stem cells for 24 and 48 hours were measured with CCK-8 method. The human hepatoma stem cell line Hep G2 was administrated by BA at concentrations of 40, 20, 10, 5 μmol/L for 48 hours, and cell cycle and apoptosis rate were measured by flow cytometry. Results BA could inhibit Hep G2 stem cell proliferation obviously with dose-effect relationship. BA influenced cell cycle, and induced tumor stem cell apoptosis. 40 μmol/L BA blocked cell cycle in S phase, and cell apoptosis rate reached 10.86%. Conclusion BA has obvious inhibitory effects on proliferation of Hep G2 liver cancer stem cell, which probably plays a part in anti-cancer by influencing cell cycle and inducing cell apoptosis.