不同产地杜仲叶红外光谱与薄层色谱特征研究

Research on Characteristics of Eucommia ulmoides Leaf from Different Producing Areas by IR and TLC

目的分析比较不同产地杜仲叶的红外光谱,建立杜仲叶的薄层色谱鉴别方法,为其质量控制提供依据。方法利用红外光谱、二阶导数谱图对6种不同产地杜仲叶进行特征峰的指认和对比分析,并进行聚类分析;通过薄层色谱对杜仲叶中活性成分进行分离和鉴别。结果通过特征峰峰形、峰位与峰强的对比,发现不同产地杜仲叶之间存在差异,聚类结果显示可将6个产地杜仲叶分为1类;薄层色谱结果显示6批样品主斑点一致,但不同产地的样品间微量成分斑点存在差异。结论 2种方法直观、简单、方便、快速,为杜仲叶的分离鉴别和质量控制提供了依据。

Objective To analyze and compare the Eucommia ulmoides leaves from different producing areas by IR; To establish a TLC method for identification of Eucommia ulmoides leaves; To provide a reference for its quality control. Methods The characteristic absorption peaks were identified and the positions and intensities of Eucommia ulmoides leaves from 6 producing areas were compared by IR and second derivative spectrum, including clustering analysis; Separation and identification of the active components in Eucommia ulmoides leaves were conducted by TLC. Results There a difference exited in the characteristic absorption peaks in peakshapes, positions and intensities. And the samples from 6 producing areas could be divided into one category. Results found 6 batches of sample spots of consistent by TLC, but between samples of different producing areas trace components spots existed differences. Conclusion The two methods are direct, simple, fast and convenient, which can provide a reliable evidence for identification and quality control of the Eucommia ulmoides leaves.