糖尿病周围神经病变大鼠模型制作的实验研究

Experimental Study on Production of Diabetic Peripheral Neuropathy Model Rats

目的:建立2型糖尿病周围神经病变(DPN)大鼠模型。方法:SD大鼠40只分为对照组(10只)和模型组(30只),模型组予高脂高糖饲料喂养及2~3次小剂量腹腔注射1%的链脲佐菌素(STZ)建立糖尿病(DM)动物模型,对照组给予普通饲料喂养并注射同等剂量生理盐水,模型组中DPN造模成功的作为DPN组,DPN造模不成功但DM造模成功的作为DM组;于实验第14周后,检测大鼠坐骨神经动作电位传导速度、血清神经生长因子(NGF)水平及坐骨神经病理形态学变化。结果:30只大鼠中DM成模24只,其中15只大鼠并发DPN,DPN成模率62.5%;与对照组相比,DPN组大鼠坐骨神经动作电位传导速度、血清NGF含量均明显降低(P<0.05);与DM组相比,DPN组大鼠血清NGF含量明显降低(P<0.05)。结论:高脂高糖饮食联合腹腔注射STZ可成功制作DPN大鼠模型,其机制与大鼠血清NGF水平降低有关。

Objective： To establish Type II diabetic peripheral neuropathy model of rats. Methods： 40 SD rats were divided into normal control group （Group A, 10） and model group （Group B, 30） ; Group B fed with high fat and sugar diet. Giving Group B 2 to 3 times of small dose of intraperitoneal injection of 1% STZ concentration and use self-made mold to form the diabetes model. Group A fed with normal diet and equal amount of saline water. Group B DPN model was established successfully and then used as DPN group; DPN modeling failed ones but DM modeling succeed ones were used as DM group. 14 weeks later, rats＇sciatic potential conduction velocity, NGF and sciatic pathomorphology was tested. Results： Out of 30 rats, 24 succeeded in DM modeling, 15 rats were complicated by DPN, DPN modeling success rate was 62.5% ; comparing with control group, rats＂ sciatic potential conduction velocity and NGF of DPN group decreased significantly （ P 〈 0.05 ） ; comparing with DM group, NGF content of DPN group decreased significantly （ P 〈 0.05 ）. Conclusion： The method can successfully induce the establishment of type 2 diabetic peripheral neuropathy model, the mechanism is correlated with rats＂ serum NGF level decrease.