不同剂量燕麦β-葡聚糖对高脂饮食诱导的大鼠血糖和血脂水平的影响

The effect of different doses of oat β-glucan on serum blood glucose and lipid of high fat induced rats

目的:研究不同剂量燕麦β-葡聚糖对高脂饮食诱导的大鼠血糖和血脂水平的影响及其相关作用机制。方法:选择50只雄性SD大鼠,按体重分层随机将其分为5组,每组10只,分别为正常对照组、高脂对照组、燕麦β-葡聚糖低剂量组、燕麦β-葡聚糖中剂量组以及燕麦β-葡聚糖高剂量组。50只雄性SD大鼠连续喂养14周后做葡萄糖耐量测试(GTT),计算血糖曲线下面积(AUC)。GTT后再连续喂养一周,将大鼠空腹处死,测定空腹血糖(FBG)、血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、游离脂肪酸(FFA)、空腹血清胰岛素(FINS)、脂联素以及胰高血糖素样肽1(GLP-1),并计算胰岛素抵抗指数(HOMA-IR)。结果:燕麦β-葡聚糖低剂量组大鼠GTT实验30 min末梢血糖高于正常对照组与高脂对照组,差异有统计学意义(95%CI分别为0.00~2.23和0.25~2.25;P<0.05);燕麦β-葡聚糖低剂量组、燕麦β-葡聚糖中剂量组大鼠GTT实验120 min末梢血糖高于正常对照组,差异有统计学意义(95%CI分别为0.82~3.57和0.28~2.90;P<0.05);燕麦β-葡聚糖高剂量组大鼠GTT检测与正常对照组和高脂对照组比较均无差异。燕麦β-葡聚糖低剂量组AUC显著高于高脂对照组,差异有统计学意义(95%CI为0.56~4.40;P<0.05),且燕麦β-葡聚糖低剂量组TC高于正常对照组与高脂对照组,差异有统计学意义(95%CI分别为0.10~0.80和0.01~0.64;P<0.05);LDL-C显著高于正常对照组与高脂对照组,差异有统计学意义(95%CI分别为0.03~0.13和0.01~0.10;P<0.05);各实验组大鼠空腹血清GLP-1与正常对照组及高脂对照组之间差异均无统计学意义。结论:低剂量的燕麦β-葡聚糖降低了实验大鼠机体对血糖的调节能力,并且增加了空腹血清TC和LDL-C水平。

Objective： To investigate the effect of different doses of oat β-glucan on serum blood glucose and lipid of high fat induced rats, and find out its related mechanism. Methods： The 50 male SD rats were divided into 5 groups： Normal control group, High fat feed group, β-glucan（low dosage） LD group, β-glucan（moderate dosage） MD group and β-glucan（high dosage） HD group. With continuous feed for 14 weeks, the glucose tolerance of the rats were detected by glucose tolerance test（GTT）, and the blood glucose area under curve（AUC） were calculated. After GTT, the rats were continuously feed for 1 week and then were euthanized. The fasting blood glucose（FBG）, serum total cholesterol（TC）, triglyceride（TG）, low density lipoprotein cholesterol（LDL-C）, high density lipoprotein cholesterol（HDL-C）, free fatty acid（FFA）, fasting serum insulin（FINS）, Adiponectin and Glucagon-like peptide 1（GLP-1） were detected, and the homeostasis model assessment of insulin resistance index（HOMA-IR） were counted. Results： The results showed that the 30 min peripheral blood glucose of β-glucan LD group was significantly higher than normal control group and high fat feed group（95% CI were 0.00~2.23 and 0.25~2.25, respectively; P〈0.05）; the 120 min peripheral blood glucose of β-glucan LD and MD groups were significantly higher than the normal control group（95% CI were 0.82~3.57 and 0.28~2.90, respectively; P〈0.05）; both of the GTT detection results between β-glucan HD group and normal control group and between HG group and high fat feed group were no significant difference（P〉0.05）, respectively. The AUC of β-glucan LD group was significantly higher than high fat feed group（95% CI was 0.56~4.40; P〈0.05）; the TC of the β-glucan LD group was higher than normal control group and high fat feed group（95%CI was 0.10~0.80 and 0.01~0.64, respectively; P〈0.05）; the LDL-C of β-glucan LD group was significantly higher than normal control group and high fat feed group（95% CI was 0.03~0.13,0.01~0.10; P〈0.05）; the serum GLP-1 of other groups were no differences with normal control group and high fat feed group（P〉0.05）. Conclusion： The low dosage of oat β-glucan can reduce the ability of blood glucose regulation function of experiment rats and increase the level of TC and LDL-C in fasting serum.