白藜芦醇对脱矿牙本质基质影响的相关性研究

Correlation Study on the Influence of Resveratrol on the Demineralized of Dentin Matrix

目的:探讨白藜芦醇对基质金属蛋白酶-2活性及耐脱矿力的影响,以期为白藜芦醇的临床应用提供实验依据。方法:设置实验组白藜芦醇的浓度梯度:12.5μmol/L、25μmol/L、50μmol/L、100μmol/L、200μmol/L、400μmol/L,阳性对照组0.2%氯己定,阴性对照组去离子水。每组取10μL与提取出来的牙源性mmp-2 30μL混合孵育30min,用体液基质金属蛋白酶-2活性比色法定量检测各组混合20 min后的酶活性,每组重复5次。牙本质片经pH循环后,用激光共聚焦电子显微镜3D图像观察牙本质脱矿深度。结果:实验组白藜芦醇浓度在50μmol/L以上时有明显抑制作用,且在100μmol/L、200μmol/L、400μmol/L时的酶活性抑制率与阳性对照组间无显著性差异。激光共聚焦电子显微镜3D图像显示100μmol/L Res的染色深度明显低于阴性对照组,统计学分析结果显示100μmol/L Res的平均脱矿深度显著低于阴性对照组(P<0.05),与阳性对照组间并无显著性差异。结论:白藜芦醇可抑制基质金属蛋白酶-2活性及牙本质脱矿。

Objective：To discuss the effects of resveratrol on matrix metalloproteinase-2（mmp-2）activity and demineralization resistance and to provide experimental evidence for the clinical application of resveratrol.Methods：The concentration gradients of resveratrol were 12.5μmol/L,25μmol/L,50μmol/L,100μmol/L,200μmol/L,and 400μmol/L in the experimental group.0.2% chlorhexidine was selected as the positive control group and deionized water as the negative control group.10μL of each group was mixed with 30μL extracted odontogenic mmp-2and incubated for 30 min.Metalloproteinase-2activity was quantitatively detected with body fluid matrix metalloproteinase-2activity assay after 20 min,and each group was repeated five times.After going through the pH cycle,electron microscopy and laser scanning confocal 3Dimage were used to observe dentin demineralization depth.Results：Resveratrol with the concentration above 50μmol/L showed significant inhibitory effect.And metalloproteinase-2activity inhibition rate in groups with the concentration of 100μmol/L,200μmol/L,and 400μmol/L showed no significant difference compared to the positive group.Confocal electron microscopy 3Dimage displayed that Res dyeing depth in the 100μmol/L group was significantly lower than that in the negative control group.And statistical analysis showed the same results（P〈0.05）.But there was no significant difference between 100μmol/L group and the positive control group.Conclusion：Resveratrol can inhibit the activity of matrix metalloproteinase-2and dentin demineralization.