汉族人群载脂蛋白CII基因二核苷酸串联重复序列(TG)n(AG)m及(AG)m多态性

Dinucleotide tandem repeats (TG)n(AG)m and (AG)m Polymorphisms of apolipoprotein CII gene in Chinese Han population

采用套式聚合酶链反应结合变性聚丙烯酰胺凝胶电泳和银染技术 ,并构建载脂蛋白CII (ApoCII)基因二核苷酸串联重复序列 (TG)n(AG)m及 (AG)m序列等位基因梯阶标准 ;检测正常汉族人群基因型和等位基因频率分布 ,检出 36种 (TG)n(AG)m序列基因型、12种等位基因。等位基因为 17、18、2 6～ 35 ,其频率分别为 0 0 6 1、0 0 11、0 0 0 2、0 0 0 2、0 0 5 4、0 2 5 5、0 372、0 0 84、0 0 2 6、0 0 39、0 0 5 2、0 0 4 1。检出 7种 (AG)m序列基因型、4种等位基因。等位基因为 6、7、8、9,其频率分别为 0 0 0 2、0 15 2、0 812、0 0 34。与欧洲白种人比较 ,ApoCII基因二核苷酸串联重复序列 (TG)n(AG)m及 (AG)m序列等位基因频率分布均具有明显的种族差异性 (P <0 0 1,P <0 0 1)。

Genetic polymorphism of apolipoprotein CII gene dinucleotide tandem repeats (TG)n(AG)m and (AG)m was analysed by using nested polymerase chain reaction and polyacrylamide gel electrophoresis. The gels were stained by silver nitrate and the genotypes of samples were determined according to the alleic ladders.36 different genotypes and 12 different alleles of apolipoprotein CII gene dinucleotide tandem repeats (TG)n(AG)m were detected in 231 healthy individuals. The allele frequency distributions were 0.061(17),0.011(18),0.002(26),0.002(27),0.054(28),0.255(29),0.372(30),0.084(31),0.026(32),0.039(33),0.052(34),0.041(35),respectively. There were 7 different genotypes and 4 different alleles of apolipoprotein CII gene dinucleotide tandem repeats (AG)m in healthy controls. The allele frequency distributions were 0.002 (6),0.152(7),0.812(8),0.034(9), respectively. Comparing with Caucasion European , the alleles frequency distributions of apolipoprotein CII gene dinucleotide tandem repeats (TG)n(AG)m and (AG)m in Han population were highly different( P <0.01, P <0.01).