Rb94基因联合放射后荷瘤裸鼠食管癌细胞的的病理改变

Pathological Change of Esophageal Carcinoma Cells of Tumor-bearing Nude Mice Combined Retinoblastoma 94 Gene with Radiation

目的探讨视网膜母细胞瘤Rb94基因与放射联合应用后裸鼠种植瘤组织的病理变化。方法将食管癌K150细胞接种裸鼠建立肿瘤模型,实验分为对照组、Ad-Lac Z组、Ad-Rb94组、照射组和Ad-Rb94联合照射组(简称联合组),观察食管癌细胞的病理改变。结果对照组和Ad-Lac Z组生长活跃,而Ad-Rb94组和照射组肿瘤细胞出现生长抑制和凋亡;与其他组相比,联合组有较少的核分裂、较浅的核深染。结论 Rb94基因与放疗联合应用对肿瘤生长具有协同抑瘤效应,显著增加食管癌治疗的疗效。

Objective To reveal pathological change of carcinoma cells of tumor- bearing nude mice combined retinoblastoma Rb94 gene with radiation. Methods The model of tumor- bearing nude mice was built by inoculating K150 cells. The cohorts were divided into five groups ： blank control、Ad- Lac Z、Ad- Rb94、radiation and Ad- Rb94 combined with radiation（ combining group for short）. The pathological change of esophageal carcinoma was detected. Results Tumors of control group and Ad- Lac Z group grew actively. Meanwhile,treatment with Ad- RB94 and / or radiation resulted in cancer cell growth inhibition and apoptosis. Compared with other ones,tumors of combination treatment group had fewer karyokinesis and lower level of nuclei hyperchromasia. Conclusion Rb94 gene combined with ionizing radiation shows synergism effect on the inhibition of tumor growth and increases apparently the curative effect on esophageal carcinoma cells.