某氡温泉对周边居民外周血淋巴细胞亚群的影响

Effects of Rdon from Hot Spring on Lymphocytes Subsets in Peripheral Blood of the Surrounding Residents

目的观察氡温泉周边居民外周血淋巴细胞亚群百分数变化,分析氡对人类免疫功能的影响。方法采用简单随机抽样法抽取某地氡温泉周边居民42人及生活习惯相似,但未接触过氡温泉的居民44人。采用流式细胞术检测两组居民外周血淋巴细胞亚群百分数(CD3^+、CD4^+CD8^-、CD4^-CD8^+,CD4^+/CD8^+,TCR/CD3)。结果氡温泉组的CD3^+(t=-4.086,P<0.01)、CD4^+CD8^-(t=-5.7,P<0.01)、TCR/CD3(t=-2.318,P<0.05)和CD4^+/CD8^+(t=-4.991,P<0.01)均值显著高于对照组。氡温泉组CD4^-CD8^+显著低于对照组,且具有统计学意义(t=3.162,P<0.01)。结论高氡温泉能够引起居民外周血淋巴细胞亚群百分数的改变,可能会影响免疫功能。

Objective To analyze the changes of immunologic functions by detecting lymphocytes subsets rate in peripheral blood of the residents surrounding with radon hot springs. Methods By random sampling methods,42 residents surrounding radon hot springs were selected as the radon group,and 44 residents with same living habit but with no contact with hot springs as control. The percentage rate of lymphocyte subsets（ CD3^＋、CD4^＋CD8^-、CD4^-CD8^＋,CD4^＋/ CD8^＋-,TCR/CD3） of the tow groups was tested by FACSAria flow cytometer among two groups. Results In radon group the averages of CD3^＋（ t =- 4.086,P〈0. 01） 、CD4^＋CD8^-（ t =- 5. 7,P〈0. 01） 、TCR/CD3（ t =- 2. 318,P〈0. 05） and CD4^＋/ CD8^＋（ t =- 4. 991,P〈0. 01） were significantly higher than those of the control group. In radon group,the CD4-CD8^＋ were significantly low（ t =3. 162,P〈0. 01）. Conclusion High radon springs could change the lymphocyte Subsets rate and probably affect immunologic functions.