侧金盏胚和胚乳发育的细胞胚胎学研究

Cytoembryological Studies in the Development of Embryo and Endosperm in Adonis amurensis Regel et Radde

利用石蜡切片技术对毛茛科植物侧金盏胚及胚乳发育进行了研究,以明确其胚胎发育的特征,为毛茛科植物的系统研究提供资料。结果表明：（1）侧金盏胚的发育属于柳叶菜型,胚乳发育为核型;初生胚乳核的分裂早于合子的第一次分裂。（2）种子成熟时,种胚尚未分化完全,尚处于球形胚后期或心形胚早期阶段,整个胚发育大约需要50-60d。（3）侧金盏种子存在明显的形态生理休眠现象,经后熟作用逐渐完成种胚的分化与生长,形成子叶形胚;侧金盏种子在相同处理条件下胚分化和发育的速度存在差异。

The experiment was conducted by means of routine paraffin-embedded sectioning technique to study the development of embryo and endosperm in Adonis amurensis,in order to define the characteristics of the embryo development and offer some information for the systematic study of Ranunculaceae plants.The results were as follows：（1）the embryogeny is of the onagrad type and the endosperm is of the nuclear type of A.amurensis.The division of primary endosperm nucleus is prior to that of zygote.（2）When the seeds have matured,the seed embryos have not been fully differentiated.They are still in the early stage of globular embryo or in the early stage of heart shaped embryo.Embryo development requires approximately50-60 d.（3）The morphophysiological dormancy exists in seeds of A.amurensis,and they need after-ripening to complete the embryos differentiation and to form cytoledon-stage embryos.Under the same condition,the differentiation rate of embryo is quite different.