自身抗体和免疫球蛋白检测对鉴别诊断原发性胆汁性肝硬化的评价

The evaluation of autoantibodies and immunoglobulin detection on the identification and diagnosis of primary biliary cirrhosis

目的:探讨自身抗体和免疫球蛋白检测在原发性胆汁性肝硬化(PBC)诊断和鉴别诊断中价值和临床意义。方法:采用间接免疫荧光法和生化检测法分别对40例PBC患者、43例病毒性肝炎患者、14例干燥综合征患者以及30例同期健康体检者进行检测,对各组患者的自身免疫性肝病指标和血清免疫球蛋白指标进行比较。结果:PBC患者的抗核抗体(ANA)、抗线粒体抗体(AMA)以及抗线粒体抗体-M2亚型(AMA-M2)阳性率明显高于其余各组,差异具有统计学意义(P<0.05);PBC患者的Ig G、Ig M水平明显高于其余各组,差异有统计学意义(P<0.05),Ig A水平差异无统计学意义(P>0.05)。结论:ANA、AMA抗体检测、免疫球蛋白检测对于PBC具有较高的临床诊断价值,值得临床推广。

Objectlve To evaluate the value and clinical significance of autoantibodies and immunoglobulin detection on the identification and diagnosis of primary biliary cirrhosis. Method 40 Cases of PBC patients ,43 viral hepatitis patients, 14 sicea syndrome patients and 30 healthy physical examination volunteers were detected by indirect immunofluorescence and biochemical assays. The autoimmune liver disease targets and serum immunoglobulin values of all groups were compared. Results The antinuclear antibodies （ ANA）, anti -mitochondrial antibody （AMA） and anti - mitochondrial antibodies - M2 subtype （ AMA - M2） positive rate of PBC patients were all significantly higher than other groups, the difference was statistically significant （ P 〈 0.05 ） . The IgG and IgM levels of PBC patients were also significantly higher than other groups （ P 〈 0. 05 ）. The difference of IgA level was not significant （ P 〉 0. 05）. Conclusion ANA, AMA antibody and immunoglobulin detection has a high clinical diagnosis value of PBC. It is worth for generalization.