TLC-HPLC-MS法检测消喘灵中非法掺入的地西泮和醋酸泼尼松

Illegal mix of diazepam and prednisone acetate in Xiao Chuan Ling identified by TLC-HPLC-MS

【目的】建立消喘灵中非法添加化学成分地西泮和醋酸泼尼松的定性检测方法。【方法】采用TLC法对怀疑掺有地西泮和醋酸泼尼松的消喘灵的提取物进行初步分离,再通过HPLC-DAD法及MS法对其进行定性。【结果】供试品中分别检测到化学成分地西泮和醋酸泼尼松。【结论】本研究提供的方法可检出中药制剂中非法掺入的化学成分地西泮和醋酸泼尼松,为药监、药检部门提供参考。

[Objective]To establish a qualitative method for identifying the illegal mix of chemical compounds diazepam and predni- sone acetate in XiaoChuanLing. [Methods] TLC was applied to preliminarily isolate the extract from XiaoChuanLing suspected of ille- gal mix of diazepam and prednisone acetate. Then HPLC-DAD and MS were used for the qualitative detection. [ Results] Diazepam and prednisone acetate were identified in the samples of XiaoChuanLing. [ Conclusions ] The detection methods in this study can iden- tify the chemical compounds diazepam and prednisone acetate illegally mixed in traditional Chinese medicine, and provide references for the governmental departments of drug surveillance and inspection..