柯萨奇病毒A10型VP1蛋白生物信息学分析

Bioinformatics apnalysis of VP1 protein of coxsackievirus A10

目的 对手足口病柯萨奇病毒A10型（CVA10） VP1蛋白进行生物信息学分析.方法 利用生物信息学软件对分离的毒株与CVA10其他基因型进行同源性比对,并构建系统进化树;预测和分析VP1蛋白的理化特性、二级结构和三级结构以及该蛋白的B细胞抗原表位.结果 JN-C10与CVA10 D型基因组同源性最高,核苷酸和氨基酸的同源性分别为91.0％-97.6％和90.4％-98.6％,属于D亚型.JN-C10 VP1基因全长为732个核苷酸,理论相对分子质量为60 580道尔顿,理论等电点为5.10,其二级结构中以无规则卷曲为主,属于胞外蛋白.以已知结构的蛋白质3vbhA为模板,构建JN-C10株VP1蛋白三级结构模型.综合多种抗原表位分析方法得出,JN-C10株VP1蛋白的B细胞抗原表位可能是13-22、101-108、120-127、169-180、213-230 5个区段等区域.结论 JN-C10毒株VP1蛋白多个可能B细胞抗原表位的预测,将为CVA10有效免疫疫苗的制备、特异性诊断系统的发展提供重要的参考依据.

Objective To analyze the biological information of the VP1 protein of coxsackievirus A10.Methods Nucleotide and amino acid homology of isolated strains were aligned with other CVA10 genotypes.Phylogenetic tree was constructed by bioinformatics software.Physicochemical properties,secondary structure and tertiary structure of VP1 protein were analyzed,and the B-cell epitopes were predicted.Results Nucleotide and amino acid homology of JN-C10 with CVA10 D were 91.0%-97.6% and 90.4%-98.6%,respectively.It was closely related to D genotypes.The full length gene of JN-C10 VP1 region was 732 nt,and theoretical molecular weight was 60580 D,theoretical isoelectric point was 5.10.The protein was a cell extracellular protein,and predominate secondary structure was random coil.The tertiary structure was constructed based on an known structures proteins 3vbhA.Through a comprehensive analysis,B-cell epitopes of JN-C10 strain VP1 protein may locate at regions of 13-22,101-108,120-127,169-180 and 213-230.Conclusions Prediction of JN-C10 B-cell epitopes will provide important information for preparing effective vaccines and developing of specific diagnostic system of CVA10.