乙型肝炎病毒对高尔基体糖蛋白-73表达的影响研究

Study on the effect of hepatitis B virus on the expression of Golgi protein 73

目的探讨乙型肝炎病毒(HBV)对高尔基体糖蛋白-73(GP73)表达的影响,并探讨其临床意义。方法采用反转录PCR和Western blot印迹法检测Hep G2和Hep G2.2.15中GP73 mRNA和蛋白的表达;将HBV感染性克隆p HBV1.3转染Hep G2细胞,反转录PCR和Western blot印迹法检测GP73 mRNA和蛋白的表达;酶联免疫吸附法(ELISA)检测对照者、慢性乙型肝炎患者和肝癌患者血清GP73的血清水平。结果 GP73在Hep G2.2.15细胞mRNA和蛋白表达水平较Hep G2细胞高;转染了HBV感染性克隆p HBV1.3的Hep G2细胞GP73 mRNA和蛋白表达较对照细胞高;与对照组相比,CHB组患者和HCC组患者的GP73血清水平显著升高,差异有统计学意义(P<0.05)。结论HBV上调GP73的表达水平,其血清学水平与HBV相关疾病进程相关。

Objective To explore the effect of hepatitis B virus（ HBV） on the expression of Golgi protein 73（ GP73） and its clinical significance. Methods mRNA and protein expression of GP73 in Hep G2 and Hep G2. 2. 15 cells were measured by RT-PCR and Western blot; HBV infectious clone p HBV1. 3 was transfected into Hep G2 cells,and mRNA and protein expression of GP73 were measured by RT-PCR and Western blot. Serum GP73 levels in healthy individuals,chronic hepatitis B（ CHB） patients and hepatocellular carcinoma（ HCC） patients were measured by enzyme-linked immunosorbent assay（ ELISA）. Results Expression of GP73 mRNA and protein were higher in Hep G2. 2. 15 cells than that in Hep G2 cells. Also,GP73 expression was higher in Hep G2 cells transfected with HBV infectious clone p HBV1. 3 than that in the control cells. As compared with healthy individuals,serum GP73 levels was much higher in CHB patients and in HCC patients,with the differences statistically significant（ P 0. 05）. Conclusion HBV upregulated the expression of GP73,and its serum levels are associated with the progression of HBV-related diseases.