2012年-2014年珠海市流行的Ⅰ型登革病毒分子溯源分析

Molecular traceability analysis of typeⅠdengue virus in Zhuhai during 2012-2014

目的对2012年-2014年珠海市流行的Ⅰ型登革病毒(DV)进行包膜蛋白基因序列测定,分析病毒可能的来源。方法采集2012年-2014年珠海登革热流行期间登革热疑似和临床诊断病例的急性期血清696份,采用荧光RT-PCR检测登革病毒核酸,选取46份DVⅠ型核酸阳性标本,用荧光RT-PCR扩增包膜蛋白基因并进行测序分型。结果荧光RT-PCR检测DV通用型核酸阳性为152例,阳性率为21.84%(152/696),其中DVⅠ型阳性为148例;选取的46份标本全部成功分型。DVⅠ型碱基同源性为89.34%~99.77%,其同源性与2014年中国广州地区、2013年中国中山地区和2011年印度的DVⅠ型流行株接近。结论 2012年-2014年珠海市DV流行主要以Ⅰ型为主,流行方式属于周边地市或东南亚国家输入性病例引起的本地暴发。

Objective To determine the envelope（ E） gene sequence of typeⅠ dengue virus（ DV） in Zhuhai during 2012-2014,and to explore their possible origins. Methods 696 samples of serum from the suspected and clinical diagnosed dengue fever cases in acute phase were collected to detect the viral nucleic acid by using real-time fluorescence RT-PCR during2012-2014. E gene of 46 typeⅠDV positive samples were amplified by RT-PCR,and the amplification products were subjected to sequence. Results 152 samples were DV nucleic acid positive by using real-time RT-PCR,of which 148 samples were typeⅠ DV nucleic acid positive,with the positive rate of 21. 84%（ 152/696）. All of 46 specimens were successfully genotyped. Nucleotide sequence homology of DVⅠwas within 89. 34%-99. 77%,whose homology was very close with DVⅠepidemic strains in 2014 in Guangzhou,2013 in Zhongshan and 2011 in India. Conclusion DVⅠwas epidemic in Zhuhai during2012-2014,and the outbreaks were caused by imported cases.