胃腺癌组织中CerbB-2、CK20及KI67的表达与淋巴结转移及远期预后的关系

Correlation study of the expression of CerbB-2, CK20, KI67 in gastric adenocarcinoma with lymph node metastasis and long-term prognosis

目的探讨CerbB-2、CK20、KI67蛋白表达与胃腺癌患者淋巴结转移及预后的关系。方法将88例原发性胃腺癌手术切除标本进行免疫组织化学方法染色，对癌组织中的CerbB-2、CK20、KI67蛋白表达进行检测，并分析CerbB-2、CK20、KI67与淋巴结转移及术后生存时间的关系。结果CerbB-2、CK20、KI67蛋白阳性表达组的淋巴结转移率分别为78.7%、78.1%、73.3%，均高于对应阴性表达组。同时有2个或3个指标表达阳性组淋巴结转移率为91.1%，明显高于1个指标表达组（56.5%）及3个指标均不表达组（15.0%），差异均有统计学意义（P〈0.01）。CerbB-2、CK20、KI67阳性表达组患者的术后生存时间明显短于CerbB-2、CK20、KI67阴性组。结论CerbB-2、CK20、KI67蛋白的表达与胃腺癌淋巴结转移及较差的预后密切相关，对分析胃癌的生物学行为和判断预后有重要意义。

ObjectiveTo investigate the correlation of the expression of CerbB-2, CK20, KI67 in gastric adenocarcinoma with the lymph node metastasis and prognosis.MethodsEighty-eight patients with primary gastric adenocarcinoma were treated by surgery. CerbB-2, CK20, KI67 were assessed by immunohistochemistry after operation, and the correlation of the lymph node metastasis with the expressions of CerbB-2, CK20, KI67 were analyzed, and the expressions of CerbB-2, CK20, KI67 with the prognosis of live time after operation were observed.ResultsIn gastric adenocarcinoma tissues, the positive rates of CerbB-2, CK20, KI67 protein were 89.1%, 78.1%, 91.0%, the expression of CerbB-2, CK20 protein had positive correlation with lymph node metastasis（P〈0.05）, KI67 had no correlation with lymph node metastasis（P〉0.05）. The lymph node metastasis rate in tissues with positive expression of two or more parameters was 91.1%, which was significantly higher than that of the tissues positive expression of one parameters. The prognosis of live time of the positive of CerbB-2, CK20, KI67 combined with the positive of lymph node metastasis was significantly shorter than that of the negative of CerbB-2, CK20, KI67 combined with the negative of lymph node metastasis.ConclusionsThe abnormal expressions of CerbB-2, CK20, KI67 is closely correlated with gastric adenocarcinoma lymph node metastasis, and is important in analyzing biological behavior and predicting the prognosis of gastric adenocarcinoma.