摘要
Cytotoxicities of nickel oxide nanoparticles（NiO NPs） with average diameter of 20 nln were investigated on cultured Chlorella vulgaris. Alga growth-inhibition tests were taken and ultrastrueture changes of the microalgae were characterized with transmission electron microscopy（TEM）. The biological interface conversion effect between NiO nanoparticles and Chlorella vulgaris was studied by X-ray diffraction（XRD）, high-resolution transmission elec- tron microscopy（HRTEM） and X-ray photoelectron spectroscopy（XPS）. The results indicated that the NiO nanopar- tides had severe inhibitory effect on the growth of microalgae, with a 96 h ECs0 value of 31.4 mg/L. Under the expo- sure to NiO NPs suspensions, ChloreIIa vulgaris cells showed plasmolysis with a shriveled cell shape, disrupted plas- ma membrane, leaked cytosol and disordered thylakoid grana lamella. The NiO NPs were aggregated and partially re- duced to Ni0 inside the Chlorella vulgaris. The bioaccumulation and bio-reduction ability of Chlorella vulgaris provide us with a possible strategy of remediation of aquatic pollution conducted by toxic metal oxide nanoparticles.
Cytotoxicities of nickel oxide nanoparticles（NiO NPs） with average diameter of 20 nln were investigated on cultured Chlorella vulgaris. Alga growth-inhibition tests were taken and ultrastrueture changes of the microalgae were characterized with transmission electron microscopy（TEM）. The biological interface conversion effect between NiO nanoparticles and Chlorella vulgaris was studied by X-ray diffraction（XRD）, high-resolution transmission elec- tron microscopy（HRTEM） and X-ray photoelectron spectroscopy（XPS）. The results indicated that the NiO nanopar- tides had severe inhibitory effect on the growth of microalgae, with a 96 h ECs0 value of 31.4 mg/L. Under the expo- sure to NiO NPs suspensions, ChloreIIa vulgaris cells showed plasmolysis with a shriveled cell shape, disrupted plas- ma membrane, leaked cytosol and disordered thylakoid grana lamella. The NiO NPs were aggregated and partially re- duced to Ni0 inside the Chlorella vulgaris. The bioaccumulation and bio-reduction ability of Chlorella vulgaris provide us with a possible strategy of remediation of aquatic pollution conducted by toxic metal oxide nanoparticles.
基金
Supported by the National Natural Science Foundation of China（No.20977012）.
