胃癌组织中HIF-1α,CD68及Sema4D蛋白的表达及临床意义

The Correlation and Clinical Significance of Expressions of HIF-1α,CD68 and Sema4D in Gastric Carcinoma

目的比较低氧诱导因子-1α(HIF-1α)、肿瘤相关巨噬细胞(TAMs)标记抗原CD68和轴突导向因子4D(Sema4D)在胃癌组织和癌旁组织中的表达,探讨三者之间的相关性及临床意义。方法采用免疫组织化学SP法检测86例胃癌组织和癌旁正常组织中HIF-1α,CD68和Sema4D的表达,并分析其与临床病理特征的关系。结果胃癌组织中HIF-1α,CD68及Sema4D蛋白阳性表达率显著高于正常组织(分别为58.1%%VS 23.3%,72.1%VS 23.3%,74.4%VS 29.1%;P均<0.01)。HIF-1α,CD68及Sema4D蛋白与胃癌组织分化类型、TNM分期和淋巴结转移明显有关(P<0.05),且三者表达均呈正相关(r HIF-1α/CD68=0.418,r HIF-1α/Sema4D=0.367,r CD68/Sema4D=0.467,P均<0.01)。结论胃癌组织中HIF-1α,CD68及Sema4D表达明显增高,且三者的表达均呈正相关,三者的联合检测可作为胃癌早期诊断的辅助指标。

Objective To compare the expressions of hypoxia-inducible factor-1 alpha（ HIF-1α）,CD68 and Semaphorin4D（ Sema4D） in gastric carcinoma and peri-carcinoma samples to investigate the correlation among them and clinical significance. Methods The expressions of HIF-1α,CD68 and Sema4 D were examined in 86 cases of gastric carcinoma and peri-carcinoma tissues by SP immunohistochemical staining. The relationship among the expressions and clinical pathological characters was analysied. Results The positive rates of HIF-1α,CD68 and Sema4 D in gastric carcinoma tissues were significant higher than those in adjacent tissues（ 58. 1% VS 23. 3%,72. 1%VS 23. 3%,74. 4% VS 29. 1%,respectively; both P〈0. 01）. HIF-1α,CD68 and Sema4 D were closely correlated with gastric carcinoma histological types,TNM stages and lymphatic metastasis（ all P〈0. 05）,and a significant correlation was observed among the expressions of HIF-1α,CD68 and Sema4 D oproteins in gastric carcinoma with the Spearman rank correlation（ r HIF-1α / CD68 = 0. 418,r HIF-1α / Sema4 D = 0.367,r CD68 / Sema4 D = 0. 467; all P〈0. 01）. Conclusion The positive rates of HIF-1α,CD68 and Sema4 D in gastric carcinoma tissues are significant increased and the expression of the three was significantly positively correlated. Combined detection of HIF-1α,CD68 and Sema4 D might be a helpful marker for the early diagnosis of gastric carcinoma.