双夏汤改善老年果蝇片段式睡眠的药效学研究

A Pharmacodynamical Study on the Improvement of Senile Drosophilas with Fragmented Sleep with the Administration of Shuang Xia Decoction

目的:本文主要探究双夏汤水煎液冻干粉对正常雌性老年果蝇片段式睡眠活动的改善作用。方法:以果蝇为模式生物,使用果蝇自主活动监测软件对果蝇的自主活动进行监测,探究双夏汤对老年果蝇片段式睡眠的调节。结果:双夏汤冻干粉的最佳给药浓度为2.5%,最佳给药天数为4天。相比于正常对照组,双夏汤给药组、阳性药组的35日龄处女蝇睡眠时间均有所延长。但阳性药主要影响其白天的睡眠行为,双夏汤则显著影响其夜晚的睡眠行为,延长片段睡眠时间。结论:双夏汤水煎液冻干粉能有效改善年老果蝇的片段式睡眠,相比于阳性药,在治疗夜间间歇性失眠的同时,并未显著影响果蝇白天的活动,双夏汤可能更有助于果蝇睡眠-觉醒节律的调节。

This study aimed at unfolding the therapeutic effects of freeze-dried powder separated from Shuang Xia decoction（SXD） on female senile drosophila melanogaster with fragmented sleep. Taking drosophilas as the model organisms, the locomotor activity was monitored using the autonomic monitoring software to explore the regulation of fragmented sleep in senile drosophilas by the treatment of SXD. As a result, it was found that the optimum concentration of SXD was 2.50%, while the desirable therapeutic duration was 4 days. In comparison with the control group, 35-day-old virgin drosophilas’ sleep was prolonged in the SXD group and the positive control group. The positive drug mainly affected their sleep in the daytime, while SXD impacted their sleep at night featuring the prolonged fragmented sleep. In conclusion, it was demonstrated that the freeze-dried powder of SXD effectively alleviated intermittent insomnia in the female senile drosophilas. Compared with positive drug, SXD also mitigated intermittent insomnia at night without significant changes in the sleep of the drosophilas in the daytime. Above all, SXD was beneficial in the regulation of sleep-wake rhythm in the drosophilas.