低离子聚凝胺在输血检验技术中的应用

Low ionic polybrene application in transfusion inspection technology

目的探讨低离子聚凝胺在输血检验技术中的应用。方法选取2013年1月~2015年10月间我院收治的受血者以及供血者400例作为研究对象,随机进行分组,各200例。对照组给予盐水配血法检测,实验组给予低离子聚凝胺检测,对比两组试验不同ABO血型供/受体间的交叉配血阳性率以及临床效果。结果不同ABO血型显示：当为A型血时实验组阳性率（94.55%）高于对照组（76.36%）,B型时实验组阳性率（97.78%）高于对照组（82.22%）,AB型时实验组阳性率（96.00%）高于对照组（84.00%）,O型时实验组阳性率（94.00%）高于对照组（80.00%）;实验组总阳性191例（95.5%）,对照组总阳性166例（83.00%）,两组间差异具有统计学意义（P〈0.05）;进行交叉配血试验时,实验组灵敏度、准确度以及稳定性均明显高于对照组,两组间差异有统计学意义（P〈0.05）。结论在输血检验技术中,采用低离子聚凝胺检测效果更为显著,其具有耗时短、阳性检出率高、灵敏度高等优势,值得在临床推广。

Objective To explore low ionic polybrene application in transfusion inspection technology. Methods 400 cases of donor and recipient as research subjects admitted in our hospital in October 2014- October 2015 were selected as the study object,were randomly grouped,200 cases in each group. The control group was given saline with blood assay,the experimental group was given a low ionic polybrene detection,the positive rate of cross-matching between ABO blood donors and recipients and clinical effect was compared between the two group. Results Different ABO blood group showed： the positive rate of experimental group when the blood type A（ 94. 55%） was higher than the control group（ 76. 36%）,the positive rate of experimental group when the blood type B（ 97. 78%） was higher than the control group（ 82. 22%）,the positive rate of experimental group when the blood type AB（ 96. 00%） was higher than the control group（ 84. 00%）,the positive rate of experimental group when the blood type O（ 94. 00%） was higher than the control group（ 80. 00%）,191（ 95. 5%） were positive in the experimental group and 166（ 83. 00%） were positive in the control group,the difference between the two groups was statistically significant（ P 0. 05）; when processed crossmatchtest,sensitivity,accuracy and stability of the experimental group are significantly higher than the control group,the difference between two groups was significant（ P 0. 05）. Conclusion In transfusion testing technique,low ionic polybrene detection effect is more significant,it has a short time-consuming,the positive detection rate,the advantages of high sensitivity and other advantages,rorthy of promotion in clinical.