14-3-3θ蛋白在鼻咽癌中的表达和临床意义

Expression and Clinical Significance of 14-3-3θ Protein in Nasopharyngeal Carcinoma

目的:探讨14-3-3θ蛋白在鼻咽癌中的表达和临床意义。方法:在72例人鼻咽癌和正常鼻咽组织中利用免疫组织化学染色法检测14-3-3θ蛋白的表达情况,并分析其表达与鼻咽癌临床病理学特征之间的关系。结果:在72例鼻咽癌和正常鼻咽组织中,14-3-3θ蛋白表达的阳性率分别为69.4%和15.2%。统计学分析表明,14-3-3θ蛋白的表达与鼻咽癌临床分级(P=0.045)、淋巴结转移(P<0.001)、肿瘤复发(P=0.041)具有显著相关性,而与鼻咽癌患者的年龄(P=0.444)、性别(P=0.795)、组织类型(P=0.468)等均无关。Spearman相关性统计分析发现,14-3-3θ蛋白高表达与鼻咽癌高临床分级(r=-0.256,P=0.030)、淋巴结转移阳性(r=0.506,P<0.001)、肿瘤复发(r=0.106,P=0.033)呈正相关,而与鼻咽癌患者的年龄、性别、组织类型等均无相关性(P>0.05)。结论:在鼻咽癌组织中,癌基因14-3-3θ在鼻咽癌组织中表达增加,并且与疾病临床分级、转移复发等密切相关。

Objective： To investigate the expression and clinical significance of 14-3-3θ protein in human nasopharyngeal carcinoma. Methods： Expression status of 14-3-3θ was detected by immunohistochemistry in 72 cases of human nasopharyngeal carcinoma and normal nasopharyngeal tissues. Expressions and correlations of 14-3-3θ were analyzed. Results： In 72 cases of nasopharyngeal carcinoma and normal nasopharyngeal tissues, the positive expressions of 14-3-3θ were 69.4 % and 15.2 %. The chi square test suggested that 14-3-3θ expression was correlated with clinical stages （P=0.045）, lymph node metastasis （P〈0.001）, recurrences （P=0.041）, while it was not correlated with age （P=0.444）, sex （P=0.795）, histologic subtype （P=0.468）. Correlation analysis suggested that 14-3-30 expressions were positively correlated with clinical stages （r= -0.256, P=0.030）, lymph node metastasis （r=0.506, P〈0.001）, recurrences （r=0.106, P=0.033）, while it was negatively correlated with age, sex, and histologic subtype （P〉0.05）. Conclusion： In human nasopharyngeal carcinoma, expressions of 14-3-3θ were increased and correlated with clinical stages and metastasis.