摘要
目的探讨miR-98对鼻咽癌细胞体外增殖和侵袭能力的影响及其可能机制。方法利用脂质体介导的miR-98 mimic(及阴性对照)转染鼻咽癌CNE-1和CNE-2细胞,CCK-8检测其体外增殖能力的改变,划痕愈合及Transwell侵袭小室实验检测鼻咽癌细胞的体外迁移及侵袭潜能变化,生物信息学软件预测miR-98可能的靶基因,并经双荧光素酶及Western blotting验证靶基因。结果 MiR-98过表达能抑制鼻咽癌CNE-1和CNE-2细胞的体外生长增殖、迁移及侵袭能力;miR-98对MTDH具有直接靶向调控作用。结论本研究表明miR-98能够靶向MTDH调控鼻咽癌细胞株的体外生长增殖及侵袭能力。
Objective To investigate the effect and mechanism of miR-98 on the proliferation and invasion in nasopharyngeal carcinoma (NPC) in vitro. Methods Liposome-mediated MiR-98 mimic and negative control were applied to transfect CNE-1 and CNE-2 cells. CCK-8 assay was used to examine the proliferation of the cells in vitro. Alterations of migration and invasion were assayed by scratches and transwell chambers. Target genes of miR-98 were predicted by online target gene prediction database. Dual luciferase reporter assay and western blotting were used to validate their binding. Results MiR-98 mimic successfully decreased capacities of growth, proliferation, migration and invasion in both CNE-1 and CNE-2 cell lines. Meanwhile, MTDH was a direct target gene of miR-98. Conclusion MiR-98 inhibits the growth, proliferation, migration and invasion of NPC cell lines by targeting MTDH.
出处
《中国耳鼻咽喉颅底外科杂志》
CAS
2016年第6期455-461,共7页
Chinese Journal of Otorhinolaryngology-skull Base Surgery
基金
国家自然科学基金(81272974
81372906
81472696
81202128)
湖南省自然科学基金(2015JJ3137)
高等学校博士学科点科研基金课题(20120162120049)
中南大学自由探索计划青年教师助推基金(2012QNZT099)
关键词
鼻咽癌
MiR-98
MTDH
增殖
侵袭
Nasopharyngeal neoplasma
MiR-98
MTDH
Proliferation
Invasion