安徽省马鞍山市分离的枸橼酸杆菌的分子分型

Genetic diversity and molecular typing of Citrobacter in Maanshan,Anhui

目的了解中国安徽省马鞍山市分离到的枸橼酸杆菌的分子流行病学特征。方法 2007 2011年从安徽省马鞍山市的腹泻患者粪便、食品和食品加工者肛拭分离到62株枸橼酸杆菌,包括13株弗氏枸橼酸杆菌、41株杨氏枸橼酸杆菌和8株布氏枸橼酸杆菌。所有菌株用XbaⅠ酶切进行脉冲场凝胶电泳(PFGE)分型和扩增7个管家基因的多位点序列分型(MLST)分析。结果 PFGE将62株枸橼酸杆菌分成43个带型(PTs),MLST将其分成53个ST型别(STs)。CITX01.CN0039是PFGE的优势带型,由2007-2008年间从食品和腹泻患者粪便分离的7株杨氏枸橼酸杆菌组成。53个ST型别分成5个ST序列群,ST序列群39个优势菌株来自腹泻患者粪便、食品和食品加工者肛拭分离的枸橼酸杆菌。从腹泻患者粪便、食品和食品加工者肛拭分离得到的枸橼酸杆菌具有相同的PTs和STs。结论在食品和人群中监测枸橼酸杆菌对预防和控制枸橼酸杆菌引起的腹泻是必要的。

Objective To understand the genetic and epidemiological relationship of Citrobacter strains isolated in Maanshan,Anhui province.Methods A total of 62 Citrobacter isolates were collected from diarrheal patients,foods and food processors in Maanshan from 2007 to 2011,including 13 C.fruendii strains,41 C.youngae strains and 8 C.braakii strains.All the isolates were analyzed to understand their genetic diversity and molecular relationships by pulsed field gel electrophoresis（PFGE） with Xba Ⅰ and multi-locus sequence typing（MLST） for 7 housekeeping genes.Results PFGE divided the 62 Citrobacter isolates into 43 pulse types（PTs） and MLST divided them into 53 sequence types（STs）.By PFGE,CITX01.CN0039 was predominant PT,which consisted of 7 C.youngae strains isolated from foods and diarrheal patients between 2007 and 2008.Related STs in 53 STs were divided into 5 ST complexes,and ST complex 39 included isolates from diarrheal patient,food processors and foods.Isolates from diarrheal patients shared the same PTs and STs with those from foods and food processors.Conclusion Surveillance for Citrobacter is important for the prevention and control of diarrheal infections caused by Citrobacter.