北京市零售鸡肉标本中弓形杆菌污染初步分析

Isolation and identification of Arcobacter from retail chicken meat in Beijing

目的了解北京市零售鸡肉标本中弓形杆菌的污染情况。方法随机购买北京地区6个农贸市场零售鸡肉样本60份,应用驱动增强双孔滤膜法对鸡肉样品进行弓形杆菌的分离培养,利用多重PCR及16S rRNA、rpo B基因测序的方法进行菌株及菌种鉴定。结果 60份零售鸡肉样本弓形杆菌的检出率为73.33%(44/60)。44份阳性标本中,共获得125株弓形杆菌分离株,其中以嗜低温弓形杆菌为主(91.20%),其次是布氏弓形杆菌(8.00%),斯氏弓形杆菌(0.80%)。两种及以上菌种混合污染率为15.00%(9/60)。结论驱动增强双孔滤膜法可有效分离肉类样本中弓形杆菌。本地区零售鸡肉样本中弓形杆菌污染严重,鸡肉中嗜低温弓形杆菌的污染高于布氏弓形杆菌。

Objective To investigate the contamination status of Arcobacter in retail chicken samples in Beijing.Methods A total of 60 retail chicken meat samples were randomly collected from 6 retail outlets in Beijing.Enhanced membrane filter technique was used to isolate Arcobacter from the chicken meat samples,multiplex-PCR was performed to identify Arcobacter spp.and the 16 S rRNA and rpo B genes were sequenced.Results Among the 60 samples,44 were Arcobacter positive（73.33%）,and 125 Arcobacter strains were isolated.Among the 125 isolates,the most frequently detected species was A.cryaerophilus（91.20%）,followed by A.butzleri（8.00%） and A.skirrowii（0.80%）.Nine samples（15.00%） were cocontaminated with more than one Arcobacter species.Conclusion Enhanced membrane filter technique could effectively isolate Arcobacter from chicken meat samples.In this area,the contamination of Arcobacter in chicken meat was serious,and the contamination rate of A.cryaerophilus was significantly higher than those of A.butzleri and A.skirrowii in chicken meat samples.