玉米光敏色素基因C的克隆与苗期光暗条件下表达分析

The Cloning of Phytochrome Gene C in Maize and the Expression Analysis in the Light and Dark Conditions at the Seeding Stage

光敏色素在植物的种子萌发、幼苗去黄化、叶片开张、茎伸长、开花等发育过程中具有重要调节作用。本研究根据玉米基因组数据库设计引物,获得ZmPHYC1和ZmPHYC2基因序列,分析其编码的氨基酸序列和结构域发现,6个不同光温敏感性玉米品种中光敏色素C基因序列未出现大片段的序列插入或缺失,仅发现少量SNP;ZmPHYC1与ZmPHYC2蛋白中含有1个GAF结构域、1个Phytochrome结构域、2个PAS结构域、1个His Kinase A结构域和1个类似组氨酸激酶的ATP激酶结构域;遗传进化树分析表明,单子叶和双子叶植物明显聚为不同的类别,并且玉米和高粱之间的同源性要高于玉米中PHYC1与PHYC2之间的同源性。利用实时荧光定量PCR技术分析ZmPHYC1和ZmPHYC2的表达模式发现,在光暗处理条件下,两个基因在不同品种间及各组织部位中表达量无显著差异。

Phytochrome plays an important role in the plant seed germination, seedling de-etiolation, leaf opened,stem elongation, flowering and other developmental stage. In our study, primers were designed by the maize genome database and the sequences of ZmPHYC1 and ZmPHYC2 were obtained. According to analysis of the encoded amino acid sequence and structural domains, we found that there were no large fragment sequences insertion or deletion in six different light and temperature sensitivity of maize varieties of phytochrome C gene sequences, only a small amount of SNP, ZmPHYC1 and ZmPHYC2 contains a GAF domains, a Phytochrome domain, two PAS domains, a His Kinase A domains and a ATP kinase domain which is similarly histidine kinase. Phylogenetic tree analysis showed that the monocots and dicots significantly clustered into different categories, and the homology between the maize and sorghum was higher than the homology between PHYC1 and PHYC2. The expression patterns of ZmPHYC1 and ZmPHYC2 between two different species an various tissue in the treatment of light and dark conditions were analyzed by real-time quantitative PCR, and found that there were no significant difference.