过表达核糖体装配调控蛋白NOG1对番茄果实大小的影响

Effects of Overexpressing Ribosome Assembly Protein NOG1 in Tomato on the Regulation of Fruit Size

作物果实大小是作物非常重要的农艺性状,直接影响经济效益。核仁G蛋白-1(NOG1)通过调控核糖体60S大亚基的装配影响细胞蛋白质的合成,而蛋白质的合成与作物果实大小有关。但目前尚不清楚NOG1是否参与调控植物果实大小的形成。为了研究NOG1是否参与调控植物果实大小,本试验构建了NOG1过表达载体(OE)转化番茄品种Micro-Tom,得到23棵T1代过表达转基因阳性苗。对T2代阳性苗和对照果实大小和重量分析的结果表明:过表达NOG1可以增加转基因番茄果实的大小和重量。这些结果说明NOG1蛋白通过控制蛋白质合成,参与了植物调控果实大小。

The fruit size of crops is an important agronomic trait, which affects the economic benefit directly in agriculture production. Nucleolus G protein-1（NOG1） was involved in assembling of 60 S ribosomal subunits which regulates protein synthesis in cytoplasm. Protein synthesis is related to fruit size. But it has not been reported whether NOG1 is involved in the regulation of plant fruit size. The 23 transgenic tomato（Solanum lycopersicum cv. Micro-Tom） plantlets over-expressed Nb NOG1 were obtained via agrobacterium mediated transformation.Analysis and comparison on weight and the size of fruits between control and T2 transgenic tomatoes lines revealed that overexpression of Nb NOG1 caused the increase and augmentation of fruit weight and size in transgenic tomatoes. These results displayed that NOG1 is involved in the modulation of fruit size via regulating protein synthesis.