疏花唇柱苣苔组培快繁及遗传稳定性分析

In vitro Rapid Propagation of Chirita laxflora and Genetic Stability Analysis

本研究以野生疏花唇柱苣苔叶片为外植体进行组培快繁研究,并利用流式细胞术对组培苗进行遗传稳定性分析。研究表明,最佳初代诱导培养基为MS 6-BA 0.5 mg/L和NAA 0.1 mg/L;最适继代增殖培养基为MS 0.5 mg/L 6-BA;最适生根培养基为1/2 MS添加15 g/L蔗糖和0.3 mg/L IBA。所获得的组培苗在无土栽培基质中移栽驯化成活率达到95%以上。组织学切片检测表明,在本实验所用激素浓度组合和培养条件下,以疏花唇柱苣苔叶片为外植体所形成芽体均为器官发生方式形成。流式细胞术检测表明组培苗倍性没有变化,但基因组大小与母本相比发生了11%的增加;母本苗和组培再生苗具有相同的染色体数目(2n=36),植株形态特征上也没有明显差异。

In vitro rapid propagation of Chirita laxflora using leaf section as explant was studied, and the genetic stability of the regenerated plantlets was analysed by flow cytometry. The results showed that the most optimum bud induction medium was MS medium supplemented with 0.5 mg/L 6-BA and 0.1 mg/L NAA; the suitable subculture medium was MS medium with 0.5 mg/L 6-BA; 1/2 MS medium supplemented with 15 g/L sucrose and0.3 mg/L IBA was suitable for rooting. The transplanting survival rate of tissue cultured seedlings in the soilless culture medium reached over 95%. Histological examine revealed that the buds and plantlets were regenerated through organogenesis in the hormone concentration combination and cultivation. Ploidy investigation by Flow cytometry showed that there was no ploidy variation among the regenerated plantlets, though there is a 11%increase in the genome size. Both the mother plant and in vitro regenerated plantlets had the same chromosome numbers（2n=36）; and there was no apparent change on morphological characteristics.