特色马铃薯品种红宝石再生系统的筛选

Screening of Regenerated System of Characteristic Potato Variety 'Hongbaoshi'

采用3因素3水平L9(34)正交试验设计,以特色马铃薯品种红宝石试管苗茎段为试材,分别选用3种植物激素(诱导愈伤用NAA,2,4-D,6-BA;诱导分化用NAA,GA3,6-BA)的3个水平对其茎段的离体再生进行研究,筛选适合红宝石的愈伤和芽分化诱导培养基。结果表明,不同培养基表现出不同的诱导效果,愈伤组织诱导的最佳培养基是MS+NAA 0.5 mg·L-1+2,4-D 1.0mg·L-1+6-BA 0.5 mg·L-1,愈伤率达100%;植株再生最佳培养基是MS+NAA 0.5 mg·L-1+GA31.0 mg·L-1+6-BA 1.0 mg·L-1,再生率为80.3%,分化后再生苗健壮。

L9 （3^4）orthogonal design was adopted to select the optimal plant hormone combination for regeneration of ‘Hongbaoshi＇ potato stem. Three plant hormones（callus induction with NAA,2,4-D,6-BA and bud differentiation with NAA ,GA3,6-BA） were applied at three different levels to study their influences on the growth and differentiation of potato stem. The results indicated that the optimal medium for callus induction from stem was the 6 number medium （MS＋NAA0.5 mg. L-1＋2.4-D1.0 mg. L-1＋6-BA0.5 mg. L-1）, with 100% induction percentage. The optimal medium for inducing bud differentiation from stem callus were the number 8 medium （MS＋NAA0.5 mg. L-1＋GA31.0 mg· L-%6-BA1.0 mg·L-1）, with 80.3% induction percentage of adventitious buds and strong seedling.