MicroRNA-21和microRNA-664减轻肾缺血再灌注损伤的研究

MicroRNA-21 and microRNA-664 relieve renal ischemia-reperfusion injury

目的探讨microRNA-21和microRNA-664对肾缺血再灌注损伤(IRI)的影响。方法应用agomir-21和agomir-664预处理BALB/c小鼠,采用双侧肾蒂阻断法复制小鼠肾IRI模型,小鼠肾缺血再灌注后24 h,采用实时荧光定量聚合酶链反应(q RT-PCR)法和Western blot检测缺血后肾组织中microRNA-21和microRNA-664及其靶基因、靶蛋白的表达,观察肾脏病理组织学的变化。结果 agomir-21和agomir-664预处理BALB/c小鼠经肾IRI后,肾脏损伤减轻,表现为肾小管上皮细胞坏死降低,进一步检测发现microRNA-21和microRNA-664的靶基因PTEN、PDCD4和MAPK表达降低,靶蛋白Caspase-3和ERK1/2表达受抑制。结论 Micro RNA-21和microRNA-664可以通过调控其靶基因、靶蛋白的表达,从而减轻肾缺血再灌注损伤。

Objective To explore the effect of microRNA-21 and microRNA-664 on renal ischemia-reperfusion injury （IRI）. Methods Agomir-21 and agomir-664 were used to pretreat BALB/c mice. The method of bilateral renal pedicle occlusion was adopted to establish the renal IRI model in mice. At the 24th h after renal IRI, the expressions of microRNA-21 and microRNA-664, and their target genes and proteins in the kidneys were detected by real-time fluorescent quantitative PCR and Western blot. The histopathologieal changes of the kidneys were observed. Results Renal damage was significantly reduced in mice pretreated with agomir-21 and agomir-664, characterized by reduced necrosis of renal tubular epithelial cells. Further study showed that the expressions of mieroRNA-21 and microRNA-664 and their target genes PTEN, PDCD4 and MAPK were decreased, the expressions of their target proteins Caspase-3 and ERK1/2 were suppressed. Conclusions MieroRNA-21 and mieroRNA-664 can reduce renal ischemia-reperfusion injury through regulation of the expressions of their target genes and proteins.