摘要
目的探讨HPV16的基因型整合状态及FHIT基因缺失在宫颈癌发生发展中的作用及相关性。方法选取44例宫颈癌、58例宫颈上皮内瘤样病变(CIN)和20例宫颈正常组织的患者,免疫组化检测宫颈FHIT蛋白的表达;并用多重PCR法检测HPV16 E2/E7表达。结果 122例单一HPV16阳性标本HPV16总整合率为78.69%,在正常宫颈组织、CIN1、CIN2、CIN3和宫颈癌中整合率分别为60.00%、66.67%、75.00%、86.96%和88.63%,随病变加重,整合率增强,组间差异有统计学意义(P<0.05);FHIT蛋白的总缺失率为43.44%,FHIT蛋白在正常宫颈组织、CIN1、CIN2、CIN3和宫颈癌中的缺失率分别为15.00%、20.00%、25.00%、43.48%和72.73%。FHIT蛋白的缺失率,随病变加重,缺失率增高,组间差异有统计学意义(P<0.05)。FHIT蛋白缺失在不同HPV16整合时不同,差异有统计学意义(P<0.05)。结论 HPV16基因整合和FHIT蛋白缺失在宫颈癌的发生、发展中起重要作用,HPV16基因整合可能通过诱导FHIT基因低表达从而促使宫颈癌发生发展。
Objective To explore the integration of human papilloma viral 16(HPV16) and its relationship with the expression of fragile histidine triad(FHIT) in cervical cancer. Methods The physical status of HPV16 E2/E7 was detected by Multiplex PCR(Polymerase chain reaction) in 44 patients with cervical cancer, 58 patients with CIN and 20 patients with normalcervix tissues. And immtmohistochemistry S-P assay was used to detect the ex- pression of FH/T before operation. Results The total integration rate of HPV16 was 78.69% in 122 cervical specimens with only HPV16 positive. The integration rates of HPV16 were 60.00%, 66.67%, 75.00%, 86.96% and 88.63%, in normal cervix tissues, CIN1,CIN2,CIN3 and cervical cancer. And it showed an increasing trend in different cervical lesions(X^2 =5.317 P=0.003〈0.05); The total descending rate of FHIT was 43.44%, the descending rates of FHIT were 15.00%20.00%, 25.00%, 43.48% and 72.73%, respectively in different cervical lesions. The difference was statistically significant (X^2 =9.931 P=0.002 〈0.05). The difference between the total integration of HPV16 and the total descending expression of FHIT was siginificant (X^2 = 11.211 P=0.002, Kruskal WaUis test). Conclusion There is a close relationship between HPV16 genome integration correlates and the descending expression of FH1T in the progression of cervical lesion.
出处
《中国妇产科临床杂志》
CSCD
北大核心
2017年第1期17-19,共3页
Chinese Journal of Clinical Obstetrics and Gynecology
基金
国家自然科学基金资助项目(81160446)
广西柳州市科技攻关资助项目(2013J030403)
柳州市培养新世纪学术和技术带头人专项资金资助项目[柳人社发(2015)141号]
关键词
人乳头状瘤病毒
脆性组氨酸三联体
整合
宫颈上皮内瘤样病变
宫颈癌
human papillomavirus (HPV)
fragile histidine triad (FHIT)
integration
cervical intraepithelialneoplasia
cervical cancer