AP-2α对滋养细胞凋亡、侵袭作用的影响

Effect of AP-2α on the apoptosis and invasion of trophoblast cells

目的研究转录因子激活蛋白-2α(Transcription factor activator protein-2α,AP-2α)对滋养细胞Be Wo凋亡、侵袭等生物学功能的影响,探讨在子痫前期(preeclampsia,PE)发生发展中的作用。方法将已构建的p IRES2-EGFP/AP-2α真核表达载体,采用瞬时转染的方法转入滋养细胞系Be Wo细胞中,应用real timePCR及Western blot方法,检测各组Be Wo细胞中AP-2αm RNA及蛋白表达;采用流式细胞仪检测细胞凋亡,Transwell法检测转染后各组细胞侵袭力。结果 AP-2α转染Be Wo细胞后AP-2αm RNA和蛋白表达量较对照组显著增加,其差异有统计学意义(P<0.05)。流式细胞仪检测细胞凋亡结果显示:与对照组比较,AP-2α转染组细胞凋亡数量明显增多,差异有统计学意义(P<0.05);Transwell检测细胞侵袭能力实验结果显示:与对照组比较,AP-2α转染组细胞侵袭的数量显著减少,差异有统计学意义(P<0.05)。结论证明AP-2α促进滋养细胞凋亡并降低其侵袭力,造成滋养细胞层浅表植入,从而为子痫前期致病机理研究提供了理论依据。

objective To investigate the effect of Transcription factor activator protein- 2 ct on the biological functions of trophoblast cell line BeWo, and to explore its possible role in the occutTence and development of preeclampsia （preeclampsia, PE）. Methods We incubated the human choriocarcinoma cell line BeWo cells in vitro, then constructed the AP-2 α eukaryofic expression vector to transfect BeWo cells. The expression of AP-2a was detected with Real time- PCR and Western blot, respectively. BeWo Cells apoptosis and invasion were determined by flow cytometry assay method and Transwell chambers detection. Results The wansfection efficiency was obviously increased after transfected with AP-2 a at 48h （P〈0.05） . After transfected with plRES2-EGFP/AP-2α, cell apoptosis rote was significantly increased （P〈0.05）. Compared with control group and empty plasmid group, the invading ability significantly decreased （P〈0.05）. Conclusion It is speculated that AP-2α might be involved in the occurrence and development of preeclampsia by promoting the excessive apoptosis of trophoblast cells and decreasing the invasive ability of trophoblast cells.