丹参酮Ⅱ_A在大鼠体肠的吸收机理

THE ABSORPTION MECHANISM OF TANSHINONⅡ_A IN RAT SMALL INTESTINE

目的 研究丹参有效成分丹参酮ⅡA 的吸收机理。方法 采用大鼠在体肠吸收模型 ,以高效液相色谱法测定丹参酮ⅡA 的量 ,根据Fick方程原理对丹参酮ⅡA 含量改变进行拟合 ,求得丹参酮ⅡA 的表观吸收速率常数Ka及吸收半衰期t1/ 2 ,探索丹参酮ⅡA 在小肠的吸收机理。结果 给予不同剂量的丹参酮ⅡA,其吸收速率Ka随浓度的增加而呈微小下降 ,吸收半衰期t1/ 2 延长 ,吸收半衰期约为 0 .6～ 1.0h。结论 丹参酮ⅡA 在大鼠胃肠道的吸收存在饱和现象 ,提示丹参酮ⅡA

OBJECTIVE To study the absorption mechanism of tanshinonⅡ A in the roots of Salvia miltiorrhiza Bge. METHODS The model of intestinal absorption was used to determine the concentration of tanshinonⅡ A in rat small intestine, the change of concentration of tanshinonⅡ A was calculated according to mechanism of the Fick equation; the apparent absorption constant ( K a) and half life of absorption ( t 1/2 ) were obtained.RESULTS With the increase of dosage of tanshinonⅡ A , K a decreased and absorption half life t 1/2 (0.6 ～1.0 h) increased gradually.CONCLUSION The transport mechanism of tanshinonⅡ A in vivo is similar to active transport or facilitated diffusion.